Evidence map›Paper›PMID 37131888›Full record

ArticleReports of biochemistry & molecular biology2023

Comparing the Biological Properties of Double-Stranded DNA Extracted from Human and Porcine Placenta and Salmon Sperm.

Anastasia Proskurina, Valeriy Nikolin, Nelly Popova, Nikolay Varaksin, Tatiana Ryabicheva, Elizaveta Ershova, Svetlana Kostyuk, Olga Leplina, Alexandr Ostanin, Elena Chernykh and 1 more

Open access · goldAbstract read
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In one paragraph

Article in Reports of biochemistry & molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Anastasia ProskurinaInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Valeriy NikolinInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Nelly PopovaInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Nikolay VaraksinJSC "Vector-Best", Koltsovo, Novosibirsk Region, 630559, Russia.
Tatiana RyabichevaJSC "Vector-Best", Koltsovo, Novosibirsk Region, 630559, Russia.
Elizaveta ErshovaResearch Centre for Medical Genetics, Moscow, 115522, Russia.
Svetlana KostyukResearch Centre for Medical Genetics, Moscow, 115522, Russia.
Olga LeplinaResearch Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia.
Alexandr OstaninResearch Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia.
Elena ChernykhResearch Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia.
Sergey BogachevInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Research Institute of Fundamental and Clinical Immunology · RUInstitute of Cytology and Genetics · RUState Research Center of Virology and Biotechnology VECTOR · RUResearch Centre for Medical Genetics · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Double-stranded fragmented extracellular DNA is a participant, inducer, and indicator of various processes occurring in the organism. When investigating the properties of extracellular DNA, the question regarding the specificity of exposure to DNA from different sources has always been raised. The aim of this study was to perform comparative assessment of biological properties of double-stranded DNA obtained from the human placenta, porcine placenta and salmon sperm. Methods: The intensity of leukocyte-stimulating effect of different dsDNA was assessed in mice after cyclophosphamide-induced cytoreduction. The stimulatory effect of different dsDNA on maturation and functions of human dendritic cells and the intensity of cytokine production by human whole blood cells was analyzed Results: Human placental DNA exhibited the strongest leukocyte-stimulating effect. DNA extracted from human and porcine placenta exhibited similar stimulatory action on maturation of dendritic cells, allostimulatory capacity, and ability of dendritic cells to induce generation of cytotoxic CD8+CD107a+ T cells in the mixed leukocyte reaction. DNA extracted from salmon sperm stimulated the maturation of dendritic cells, while having no effect on their allostimulatory capacity. DNA extracted from human and porcine placenta was shown to exhibit a stimulatory effect on cytokine secretion by human whole blood cells. The observed differences between the DNA preparations can be caused by the total methylation level and are not related to differences in oxidation level of DNA molecules. Conclusions: Human placental DNA exhibited the maximum combination of all biological effects.

Indexed as

CytokinesCytotoxic T cellsDendritic cellsDouble-stranded DNA

Identifiers

PMID37131888
PMCPMC10149128
OpenAlexW4367593264

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.