Evidence map›Paper›PMID 37131694›Full record

ArticlebioRxiv : the preprint server for biology2023

Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis.

Gerald Coulis, Diego Jaime, Christian Guerrero-Juarez, Jenna M Kastenschmidt, Philip K Farahat, Quy Nguyen, Nicholas Pervolarakis, Katherine McLinden, Lauren Thurlow, Saba Movahedi and 24 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

34 authors at 9 institutions in 2 countries.

Gerald CoulisDepartment of Physiology and Biophysics, University of California Irvine, USA.
Diego JaimeDepartment of Physiology and Biophysics, University of California Irvine, USA.
Christian Guerrero-JuarezDepartment of Mathematics, University of California Irvine, USA.
Jenna M KastenschmidtDepartment of Physiology and Biophysics, University of California Irvine, USA.
Philip K FarahatDepartment of Physiology and Biophysics, University of California Irvine, USA.
Quy NguyenDepartment of Biological Chemistry, University of California Irvine, USA.
Nicholas PervolarakisDepartment of Biological Chemistry, University of California Irvine, USA.
Katherine McLindenDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, USA.
Lauren ThurlowDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, USA.
Saba MovahediDepartment of Physiology and Biophysics, University of California Irvine, USA.
Jorge DuarteDepartment of Physiology and Biophysics, University of California Irvine, USA.
Andrew SornDepartment of Physiology and Biophysics, University of California Irvine, USA.
Elizabeth MontoyaDepartment of Physiology and Biophysics, University of California Irvine, USA.
Izza MozaffarDepartment of Physiology and Biophysics, University of California Irvine, USA.
Morgan DraganDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, USA.
Shivashankar OthyDepartment of Physiology and Biophysics, University of California Irvine, USA.
Trupti JoshiDepartment of Health Management and Informatics, University of Missouri, Columbia, USA.
Chetan P HansDepartment of Cardiovascular Medicine, University of Missouri, Columbia, USA.
Virginia KimonisDepartment of Pediatrics, University of California Irvine, USA.
Adam L MacLeanDepartment of Quantitative and Computational Biology, University of Southern California, Los Angeles, USA.
Qing NieDepartment of Mathematics, University of California Irvine, USA.
Lindsay M WallaceCenter for Gene Therapy, The Abigail Wexner Research Institute at Nationwide Children's Hospital.
Scott Q HarperDepartment of Pediatrics, The Ohio State University, Columbus, OH, USA.
Tahseen MozaffarDepartment of Neurology, University of California Irvine, USA.
Marshall W HogarthChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.
Surajit BhattacharyaChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.
Jyoti K JaiswalChildren's National Hospital, Research Center for Genetic Medicine, Washington, DC, USA.
David R GolannRegeneron Pharmaceuticals, Inc., Tarrytown, NY, USA.
Qi SuRegeneron Pharmaceuticals, Inc., Tarrytown, NY, USA.
Kai KessenbrockDepartment of Biological Chemistry, University of California Irvine, USA.
Michael StecRegeneron Pharmaceuticals, Inc., Tarrytown, NY, USA.
Melissa J SpencerDepartment of Neurology, University of California Los Angeles, USA.
Jesse R ZamudioDepartment of Molecular Cell and Developmental Biology, University of California Los Angeles, USA.
S Armando VillaltaDepartment of Physiology and Biophysics, University of California Irvine, USA.
University of California, Irvine · USUniversity of California, Los Angeles · USChildren's National · USRegeneron (United States) · USNationwide Children's Hospital · USUniversity of Illinois Urbana-Champaign · USUniversity of Missouri · USUniversity of Missouri Health System · USUniversity of Southern California · US

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Training and education CoreU54AR052646 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI MCNALLY, ELIZABETH M, SWEENEY, H LEE · 2005 to 2020
$24.1M
Institutional Career Development CoreKL2TR001416 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI CAIOZZO, VINCENT JAMES, DEMETRIOU, MICHAEL · 2015 to 2023
$5.6M
UCI P30 Skin Center Systems Biology CoreP30AR075047 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2019 to 2025
$5.1M
Influence of NT5c1A antibodies on disease progression, clinical phenotype and blood and muscle biomarkers in sporadic Inclusion Body Myositis - A prospective evaluationR01AR078340 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MOZAFFAR, TAHSEEN · 2021 to 2025
$4.2M
Tissue-Specific Mechanisms of Regulatory T Cells in the CNS during Autoimmune EncephalomyelitisR01AI168063 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Shivashankar Othy · 2022 to 2026
$2.5M
Novel macrophage and regulatory T cell interactions that promote the pathogenesis of Duchenne muscular dystrophyR01NS120060 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI VILLALTA, SERGIO ARMANDO · 2020 to 2024
$2.5M
UCI-NEXT, a NeuroNEXT siteU24NS107210 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MOZAFFAR, TAHSEEN · 2018 to 2022
$1.5M
Mechanisms of Genomic Stability by Mammalian Argonaute ProteinsR01GM143536 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZAMUDIO, JESSE R. · 2021 to 2024
$1.3M
Tolerization of dystrophin and adeno-associated virus immunityR21NS114918 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI VILLALTA, SERGIO ARMANDO · 2020 to 2020
$431k
NCATS NIH HHS KL2 TR001416NCI NIH HHS P30 CA062203NIAID NIH HHS R01 AI168063NIAMS NIH HHS P30 AR075047NIAMS NIH HHS R01 AR078340NIAMS NIH HHS U54 AR052646NIGMS NIH HHS R01 GM143536NINDS NIH HHS R01 NS120060NINDS NIH HHS R21 NS114918NINDS NIH HHS U24 NS107210
6 · The paper itself

Abstract

The monocytic/macrophage system is essential for skeletal muscle homeostasis, but its dysregulation contributes to the pathogenesis of muscle degenerative disorders. Despite our increasing knowledge of the role of macrophages in degenerative disease, it still remains unclear how macrophages contribute to muscle fibrosis. Here, we used single-cell transcriptomics to determine the molecular attributes of dystrophic and healthy muscle macrophages. We identified six novel clusters. Unexpectedly, none corresponded to traditional definitions of M1 or M2 macrophage activation. Rather, the predominant macrophage signature in dystrophic muscle was characterized by high expression of fibrotic factors, galectin-3 and spp1. Spatial transcriptomics and computational inferences of intercellular communication indicated that spp1 regulates stromal progenitor and macrophage interactions during muscular dystrophy. Galectin-3

Identifiers

PMID37131694
PMCPMC10153153
OpenAlexW4366425368

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.