Evidence map›Paper›PMID 37131625›Full record

ArticlebioRxiv : the preprint server for biology2023

Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs.

Ru Zheng, Rigumula Wu, Yuanchang Liu, Zhining Sun, Yousef Bagheri, Zhaolin Xue, Lan Mi, Qian Tian, Raymond Pho, Sidrat Siddiqui and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 1 institution in 2 countries.

Ru ZhengDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Rigumula WuDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Yuanchang LiuDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Zhining SunDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Yousef BagheriDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Zhaolin XueDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Lan MiDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Qian TianDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Raymond PhoDepartment of Chemical Engineering, University of Massachusetts, Amherst, MA 01003, USA.
Sidrat SiddiquiDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Kewei RenDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Mingxu YouDepartment of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
University of Massachusetts Amherst · US

Funding

Chemistry-Biology Interface Predoctoral Training GrantT32GM139789 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ERIC Robert STRIETER · 2021 to 2026
$3.4M
NIGMS NIH HHS T32 GM139789
6 · The paper itself

Abstract

Single-cell detection of multiple target analytes is an important goal in cell biology. However, due to the spectral overlap of common fluorophores, multiplexed fluorescence imaging beyond two-to-three targets inside living cells remains a technical challenge. Herein, we introduce a multiplexed imaging strategy that enables live-cell target detection via sequential rounds of imaging-and-stripping process, which is named as "sequential Fluorogenic RNA Imaging-Enabled Sensor" (seqFRIES). In seqFRIES, multiple orthogonal fluorogenic RNA aptamers are genetically encoded inside cells, and then the corresponding cell membrane permeable dye molecules are added, imaged, and rapidly removed in consecutive detection cycles. As a proof-of-concept, we have identified in this study five

Identifiers

PMID37131625
PMCPMC10153257
OpenAlexW4366771931

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.