ArticleScientific reports2023
Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 13 citations in OpenAlex.
- Conserved and Divergent Phytochemical Profiles in Native and MicropropagatedPlants (Basel, Switzerland) · 2025Article
- Accumulation of Phenolic Compounds in Microshoot Cultures ofInternational journal of molecular sciences · 2025Article
- Adventitious and Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Elicited Production of Essential Oil with Immunomodulatory Activity inMolecules (Basel, Switzerland) · 2025Article
- Enhanced bioproduction of phenolic compounds fromFrontiers in molecular biosciences · 2025Article
- Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In VitroMolecules (Basel, Switzerland) · 2024Article
- Temporary Immersion System for Biomass Production of Salvia spp.: A Mini-Review.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Chemical Composition and Antimicrobial Activity against theFoods (Basel, Switzerland) · 2023Article
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10 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Microshoot cultures of the North American endemic Salvia apiana were established for the first time and evaluated for essential oil production. Stationary cultures, grown on Schenk-Hildebrandt (SH) medium, supplemented with 0.22 mg/L thidiazuron (TDZ), 2.0 mg/L 6-benzylaminopurine and 3.0% (w/v) sucrose, accumulated 1.27% (v/m dry weight) essential oil, consisting mostly of 1,8-cineole, β-pinene, α-pinene, β-myrcene and camphor. The microshoots were adapted to agitated culture, showing biomass yields up to ca. 19 g/L. Scale-up studies demonstrated that S. spiana microshoots grow well in temporary immersion systems (TIS). In the RITA bioreactor, up to 19.27 g/L dry biomass was obtained, containing 1.1% oil with up to ca. 42% cineole content. The other systems employed, i.e. Plantform (TIS) and a custom made spray bioreactor (SGB), yielded ca. 18 and 19 g/L dry weight, respectively. The essential oil content of Plantform and SGB-grown microshoots was comparable to RITA bioreactor, however, the content of cineole was substantially higher (ca. 55%). Oil samples isolated from in vitro material proved to be active in acetylcholinesterase (up to 60.0% inhibition recorded for Plantform-grown microshoots), as well as hyaluronidase and tyrosinase-inhibitory assays (up to 45.8 and 64.5% inhibition observed in the case of the SGB culture).
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