ArticleInfectious diseases and therapy2023
Real-Life Experience and Diagnostic Utility of the BioFire Joint Infection PCR Panel in Bone and Joint Infections: Analysis of a Prospective Validation Study.
Article in Infectious diseases and therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 16 citations in OpenAlex.
- From serum inflammatory markers to fluid, tissue, and molecular assays: current advances in the laboratory diagnosis of bone and joint infections.Frontiers in cellular and infection microbiology · 2026Review
- Clinical performance of a syndromic panel for direct identification of pathogens and antimicrobial resistance markers in pediatric osteoarticular and pleural space infections.Journal of clinical microbiology · 2025Article
- Performance and potential utility of the BioFire Joint Infection Panel with synovial fluid and joint tissue specimens.Journal of clinical microbiology · 2025Article
- High sensitivity and enhanced antibiotic stewardship of the BioFire Joint Infection Panel in acute, but not chronic, prosthetic joint infection of the knee.Microbiology spectrum · 2025Article
- Article
- Haemophilus influenzae Acute Prosthetic Joint Infection: A Rare Case Report on Time-Critical, Pathogen-Specific Management.Journal of orthopaedic case reports · 2025Article
- BioFire in osteoarticular Infections: Rapid syndromic testing for early and accurate diagnosis - A narrative review.Journal of clinical orthopaedics and trauma · 2025Review
- Performance evaluation of a commercial multiplex pathogen panel for the diagnosis of pediatric joint infections.Journal of clinical microbiology · 2025Article
- Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case.Diagnostics (Basel, Switzerland) · 2024Article
- BioFireAntibiotics (Basel, Switzerland) · 2024Article
- Clinical evaluation of a multiplex PCR-based test for joint infection: a prospective diagnostic accuracy study of forty-nine patients.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2024Article
- Clinical performance evaluation of the BioFire Joint Infection Panel.Journal of clinical microbiology · 2024Article
- Application of a metatranscriptomics technology, CSI-Dx, for the detection of pathogens associated with prosthetic joint infections.Scientific reports · 2024Article
- Diagnosis and Treatment of Acute Periprosthetic Infections with the BioFireBiomedicines · 2024Review
- Evaluation of a BioFire multiplex PCR panel for detection of joint infections using retrospective and prospectively collected specimens.Journal of clinical microbiology · 2024Article
- Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study.Journal of bone and joint infection · 2024Article
- Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre.Antibiotics (Basel, Switzerland) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMicrobiological diagnosis is central for adequate treatment of bone and joint infections. Culture-based methods have a limited diagnostic sensitivity and a long turnaround time (TAT). The objective of this study was to compare the diagnostic performance of BioFire Joint Infection Panel Investigational Use Only version (hereafter BioFire)-a sample-to-result multiplex PCR panel-with culture-based methods and 16S ribosomal RNA (rRNA) PCR and sequencing, when available.
methodsThis study presents a retrospective analysis of a prospective validation study of the BioFire panel. Specimens were obtained from consecutive patients evaluated for suspected bone and joint infections and processed using culture, BioFire, and 16S rRNA PCR and sequencing. Final clinical diagnosis was used as the reference for definition of infection.
resultsSamples, including synovial fluid, bone and periarticular tissue, were obtained from 57 patients, 39 of whom were finally diagnosed with a bone or joint infection. Cultures were positive in 27/39 infected patients and in 3/18 uninfected patients (sensitivity 69%, specificity 83%). BioFire was positive in 22/39 infected patients and in none of the uninfected patients (sensitivity 56%, specificity 100%). Sensitivity for PCR panel organisms was 92% (22/24) and sensitivity for organisms identified by any microbiological modality was 69% (22/32). Gram stain results were positive in 13/39 infected patients and in none of the uninfected patients (sensitivity 33%, specificity 100%). 16S rRNA was positive in 20/28 infected patients and in 0/12 uninfected patients (sensitivity 71%, specificity 100%). Net machine time for BioFire-1 h-was shorter than the mean TAT for Gram stain results, which was 4 h.
conclusionBioFire offered equivalent diagnostic performance with superior TAT for bone and joint infections, compared with conventional methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.