ArticleMacromolecular bioscience2023
Large Polyacrylamide Hydrogels for Large-Batch Cell Culture and Mechanobiological Studies.
Article in Macromolecular bioscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.Cell biomaterials · 2026Article
- Protocol for synthesis and mechanical characterization of polyacrylamide hydrogels of varying stiffness for cell culture applications.STAR protocols · 2026Article
- Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics.Molecular & cellular proteomics : MCP · 2026Article
- Article
- Geometrically-engineered human motor assembloids-on-a-chip for neuromuscular interaction readout and hypoxia-driven disease modeling.Nature communications · 2025Article
- Advancements in Hydrogels: A Comprehensive Review of Natural and Synthetic Innovations for Biomedical Applications.Polymers · 2025Review
- Considerations and implications of currentFrontiers in cell and developmental biology · 2025Review
- Article
- DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms.FEBS open bio · 2024Article
- Recent Progress of In Vitro 3D Culture of Male Germ Stem Cells.Journal of functional biomaterials · 2023Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The rigidity of a cell's substrate or extracellular matrix plays a vital role in regulating cell and tissue functions. Polyacrylamide (PAAm) hydrogels are one of the most widely used cell culture substrates that provide a physiologically relevant range of stiffness. However, it is still arduous and time-consuming to prepare PAAm substrates in large batches for high-yield or multiscale cell cultures. In this communication, a simple method to prepare PAAm hydrogels with less time cost and easily accessible materials is presented. The hydrogel is mechanically uniform and supports cell culture in a large batch. It is further shown that the stiffness of the hydrogel covers a large range of Young's modulus and is sensed by cells, regulating various cell features including changes in cell morphology, proliferation, and contractility. This method improves the reproducibility of mechanobiology studies and can be easily applied for mechanobiology research requiring large numbers of cells or experimental groups.
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Registered trials
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