Evidence map›Paper›PMID 37127654›Full record

ArticleScientific reports2023

A local translation program regulates centriole amplification in the airway epithelium.

Helu Liu, Huijun Li, Zhihua Jiang, Shibo Jin, Rui Song, Ying Yang, Jun Li, Jingshu Huang, Xiaoqing Zhang, Xuesong Dong and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Helu Liu *The Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Huijun Li *The Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Zhihua JiangThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Shibo JinDivision of Cellular and Developmental Biology, Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
Rui SongDivision of Cellular and Developmental Biology, Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
Ying YangThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Jun LiThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Jingshu HuangThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Xiaoqing ZhangThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Xuesong DongThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Munemasa MoriThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA.
Marvin J FritzlerDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Lin HeDivision of Cellular and Developmental Biology, Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
Wellington V CardosoThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA. wvc2104@cumc.columbia.edu.
Jining LuThe Columbia Center for Human Development, Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Columbia University Medical Center, Columbia University, College of Physicians & Surgeons, 650 West 168th Street, BB 8-812, New York, NY, 10032, USA. jining.lu@nih.gov.

Funding

Mechanisms Controlling Expansion and Lineage Specification of Airway Progenitors in Development and DiseaseR35HL135834 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI CARDOSO, WELLINGTON V. · 2017 to 2023
$6.7M
Regulation of Progenitor Cell Plasticity in Lung Development and Disease-RepairR35HL166661 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Wellington V. Cardoso · 2023 to 2026
$4.4M
Local translation and viral infection in the airway epitheliumR01AI170904 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Wellington V. Cardoso · 2023 to 2026
$2.6M
Regulatory Mechanisms of Cell Competition in Lung RegenerationR01HL148223 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MORI, MUNEMASA · 2019 to 2022
$2.1M
The Trinucleotide Repeat Containing 6a-Mediated miRNA Activities in the Ciliogenesis of Airway EpitheliumR01HL131422 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI CARDOSO, WELLINGTON V., LU, JINING · 2016 to 2019
$1.6M
NHLBI NIH HHS R01 HL131422NHLBI NIH HHS R01 HL148223NHLBI NIH HHS R35 HL135834NHLBI NIH HHS R35 HL166661NIAID NIH HHS R01 AI170904
6 · The paper itself

Abstract

Biogenesis of organelles requires targeting of a subset of proteins to specific subcellular domains by signal peptides or mechanisms controlling mRNA localization and local translation. How local distribution and translation of specific mRNAs for organelle biogenesis is achieved remains elusive and likely to be dependent on the cellular context. Here we identify Trinucleotide repeat containing-6a (Tnrc6a), a component of the miRNA pathway, distinctively localized to apical granules of differentiating airway multiciliated cells (MCCs) adjacent to centrioles. In spite of being enriched in TNRC6A and the miRNA-binding protein AGO2, they lack enzymes for mRNA degradation. Instead, we found these apical granules enriched in components of the mRNA translation machinery and newly synthesized proteins suggesting that they are specific hubs for target mRNA localization and local translation in MCCs. Consistent with this, Tnrc6a loss of function prevented formation of these granules and led to a broad reduction, rather than stabilization of miRNA targets. These included downregulation of key genes involved in ciliogenesis and was associated with defective multicilia formation both in vivo and in primary airway epithelial cultures. Similar analysis of Tnrc6a disruption in yolk sac showed stabilization of miRNA targets, highlighting the potential diversity of these mechanisms across organs.

Indexed as

CentriolesMicroRNAsEpitheliumProteinsRNA, MessengerMicroRNAsProteinsRNA, Messenger

Identifiers

PMID37127654
PMCPMC10151349

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.