ReviewJournal of mammary gland biology and neoplasia2023
Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol.
Review in Journal of mammary gland biology and neoplasia, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Integrated Single-Nucleus Multi-Omics Atlases Reveal Lineage Plasticity and Regulatory Networks of Luminal Epithelial Cells During Mammary Gland Lactation and Involution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Effects of circadian rhythms on antimicrobial peptide concentrations in lactating goat milk.BMC veterinary research · 2026Article
- The Effects of Supplemented Conjugated Linoleic Acid on Lipid Metabolism in Cattle.Animals : an open access journal from MDPI · 2026Review
- Personalized Models of Biological Barriers and Their Diseases: Recent Progress with Organs-On-Chips.Advanced biology · 2026Review
- A novel, cost-effective approach to cell-cultured milk production: Progesterone and MMP inhibition promote lactogenic differentiation in bovine mammary epithelial cells.Food chemistry: X · 2026Article
- Mammary Epithelial Cell Spheroid: Stabilization Through Vascular-Wall Mesenchymal Stem Cells and Endothelial Cells Co-Culture.Animals : an open access journal from MDPI · 2025Article
- Effects of Chestnut Tannin Extract on Enteric Methane Emissions, Blood Metabolites and Lactation Performance in Mid-Lactation Cows.Animals : an open access journal from MDPI · 2025Article
- EM Dipeptide Enhances Milk Protein Secretion: Evidence from Integrated Metabolomic and Transcriptomic Analysis.Metabolites · 2025Article
- Role of CORO1A in Regulating Immune Homeostasis of Mammary Glands and Its Contribution to Clinical Mastitis Development in Dairy Cows.Biomolecules · 2025Article
- Differential expression ofVeterinary world · 2025Article
- HCAR2 Modulates the Crosstalk between Mammary Epithelial Cells and Macrophages to Mitigate Staphylococcus aureus Infection in the Mouse Mammary Gland.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Selenium Counteracts Tight Junction Disruption and Attenuates the NF-κB-Mediated Inflammatory Response in Staphylococcus aureus-Infected Mouse Mammary Glands.Biological trace element research · 2025Article
- Phenylalanine modulates casein synthesis in bovine mammary epithelial cells by influencing amino acid transport and protein synthesis pathways.Frontiers in nutrition · 2025Article
- Cellular characteristics and milk component productivity of primary bovine mammary cells for cell-cultured milk component production.Journal of animal science and technology · 2025Article
- Effects of added exogenous hormones on lactation-related physiological functions of equine mammary epithelial cells.Frontiers in veterinary science · 2025Article
- Non-Targeted Metabolomics of White Rhinoceros Colostrum and Its Changes During Early Lactation byMetabolites · 2024Article
- Transcriptome analysis of mammary epithelial cell between Sewa sheep and East FriEsian sheep from different localities.BMC genomics · 2024Article
- Colostrum-derived extracellular vesicles: potential multifunctional nanomedicine for alleviating mastitis.Journal of nanobiotechnology · 2024Article
- Involvement of adhesins (EcpD, FdeC, FimH) expressed in mammary pathogenic Escherichia coli on adhesion to bovine mammary epithelial cells.Antonie van Leeuwenhoek · 2024Article
- Generation of Anti-Mastitis Gene-Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2-TnpB System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammary epithelial cells (MECs) are the only cell type that produces milk during lactation. MECs also form less-permeable tight junctions (TJs) to prevent the leakage of milk and blood components through the paracellular pathway (blood-milk barrier). Multiple factors that include hormones, cytokines, nutrition, and temperature regulate milk production and TJ formation in MECs. Multiple intracellular signaling pathways that positively and negatively regulate milk production and TJ formation have been reported. However, their regulatory mechanisms have not been fully elucidated. In addition, unidentified components that regulate milk production in MECs likely exist in foods, for example plants. Culture models of functional MECs that recapitulate milk production and TJs are useful tools for their study. Such models enable the elimination of indirect effects via cells other than MECs and allows for more detailed experimental conditions. However, culture models of MECs with inappropriate functionality may result in unphysiological reactions that never occur in lactating mammary glands in vivo. Here, I briefly review the physiological functions of alveolar MECs during lactation in vivo and culture models of MECs that feature milk production and less-permeable TJs, together with a protocol for establishment of MEC culture with functional TJ barrier and milk production capability using cell culture inserts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.