ArticleJournal of the American Chemical Society2023
Orthogonal Light-Activated DNA for Patterned Biocomputing within Synthetic Cells.
Article in Journal of the American Chemical Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Spatially Encoded Protocell Network for Non-Cascaded Parallel Biocomputation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- DNA-Based Boolean Logic Gates for Molecular Computation and Biosensing: A Critical Review.Molecular biotechnology · 2026Review
- Toward Chromoselective Transformations in Biological Systems: Perspectives and Challenges.Angewandte Chemie (International ed. in English) · 2026Review
- Let There be Light! Light as an Engine and Regulator in Synthetic Cells.Angewandte Chemie (International ed. in English) · 2026Review
- Review
- Low-Cost Open Platform Digital Light Printer (OP-DLP) for 96-Well Format Hydrogel Printing and Localized Light-Activation.ACS biomaterials science & engineering · 2026Article
- NIR-Activatable, Sequence-Specific Metal-Nucleic Acid Scaffolds for Responsive Uncaging.Angewandte Chemie (International ed. in English) · 2025Article
- Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification.Nature communications · 2025Article
- DNA computing function switching by programming base stacking interactions with minimal molecular architecture changes.Nature communications · 2025Article
- Genetically Encoded Control ofACS synthetic biology · 2025Article
- Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye.Angewandte Chemie (International ed. in English) · 2025Article
- Adaptive metal ion transport and metalloregulation-driven differentiation in pluripotent synthetic cells.Nature chemistry · 2025Article
- Light-Based Juxtacrine Signaling Between Synthetic Cells.Small science · 2025Article
- Nucleated synthetic cells with genetically driven intercompartment communication.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reversible Control of Gene Expression by Guest-Modified Adenosines in a Cell-Free System via Host-Guest Interaction.Journal of the American Chemical Society · 2024Article
- Recent progress in stimuli-responsive DNA-based logic gates: Design, working principles and biological applications.Smart molecules : open access · 2024Review
- Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements.Scientific reports · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Cell-free gene expression is a vital research tool to study biological systems in defined minimal environments and has promising applications in biotechnology. Developing methods to control DNA templates for cell-free expression will be important for precise regulation of complex biological pathways and use with synthetic cells, particularly using remote, nondamaging stimuli such as visible light. Here, we have synthesized blue light-activatable DNA parts that tightly regulate cell-free RNA and protein synthesis. We found that this blue light-activated DNA could initiate expression orthogonally to our previously generated ultraviolet (UV) light-activated DNA, which we used to generate a dual-wavelength light-controlled cell-free AND-gate. By encapsulating these orthogonal light-activated DNAs into synthetic cells, we used two overlapping patterns of blue and UV light to provide precise spatiotemporal control over the logic gate. Our blue and UV orthogonal light-activated DNAs will open the door for precise control of cell-free systems in biology and medicine.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.