Evidence map›Paper›PMID 37124861›Full record

ArticleIranian journal of microbiology2022

Comparison among plaque assay, tissue culture infectious dose (TCID

Maria Isabel Zapata-Cardona, Lizdany Flórez-Álvarez, Diana Maryory Gómez-Gallego, María Juliana Moncada-Díaz, Juan Carlos Hernandez, Francisco Díaz, María Teresa Rugeles, Wbeimar Aguilar-Jiménez, Wildeman Zapata

Open access · diamondAbstract read
In one paragraph

Article in Iranian journal of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Maria Isabel Zapata-CardonaGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Lizdany Flórez-ÁlvarezGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Diana Maryory Gómez-GallegoGrupo Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellín, Colombia.
María Juliana Moncada-DíazPrograma de Estudio y Control de Enfermedade Tropicales (PECET), Universidad de Antioquia UdeA, Medellín, Colombia.
Juan Carlos HernandezGrupo Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellín, Colombia.
Francisco DíazGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
María Teresa RugelesGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Wbeimar Aguilar-JiménezGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Wildeman ZapataGrupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Universidad de Antioquia · COUniversidad Cooperativa de Colombia · CO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Objectives: SARS-CoV-2 variants of concern (VOC) and interest (VOI) pose a significant threat to public health because the rapid change in the SARS-CoV-2 genome can alter viral phenotypes such as virulence, transmissibility and the ability to evade the host response. Hence, SARS-CoV-2 quantification techniques are essential for timely diagnosis and follow-up. Besides, they are vital to understanding viral pathogenesis, antiviral evaluation, and vaccine development. Materials and Methods: Five isolates of SARS-CoV-2: D614G strain (B.1), three VOC (Alpha, Gamma and Delta), and one VOI (Mu) were used to compare three techniques for viral quantification, plaque assay, median tissue culture infectious dose (TCID Results: Plaque assay showed viral titers between 0.15 ± 0.01×10 Conclusion: TCID

Indexed as

Median tissue culture infectious dose assayPlaque assayReal-time reverse transcription-polymerase chain reactionSARS-CoV-2 variantsVirus titer

Identifiers

PMID37124861
PMCPMC10132340
OpenAlexW4283206141

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.