ArticleiScience2023
General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis.
Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- Cognitive Changes in Laparoscopic Cholecystectomy: Cognitive Assessments in Decision-Making Matters.Journal of clinical medicine · 2026Article
- Pre-analytical Best Practices for RNA Sequencing from Small Biopsies and Cytologic Specimens.Molecular diagnosis & therapy · 2026Review
- Intermittent Propofol Exposure Induces Neurodevelopmental Alterations in Human Brain Organoids.Cellular and molecular neurobiology · 2026Article
- Neonatal propofol exposure impairs synaptic plasticity and cognition, associated with BAG3 upregulation and disrupted synaptic protein homeostasis.Frontiers in neuroscience · 2026Article
- Repeated sevoflurane exposure causes hypomyelination in the prefrontal cortex of adult male mice.Scientific reports · 2025Article
- Advances in research on propofol-induced postoperative cognitive dysfunction via Piezo channels.Frontiers in molecular neuroscience · 2025Review
- Comprehensive Analysis of Bulk RNA-Seq and Single-Cell RNA-Seq Data Unveils Sevoflurane-Induced Neurotoxicity Through SLC7A11-Associated Ferroptosis.Journal of cellular and molecular medicine · 2024Article
- Single-Nucleus Transcriptomic Taxonomy of Multiple Sevoflurane-Induced Cell Type Specificity in the Hippocampus of Juvenile Non-human Primates.Neuroscience bulletin · 2024Article
- Article
- GABA system as the cause and effect in early development.Neuroscience and biobehavioral reviews · 2024Review
- Effect of General Anesthetic Agents on Microglia.Aging and disease · 2024Review
- Inhibitory neuron map of sevoflurane induced neurotoxicity model in young primates.Frontiers in cellular neuroscience · 2023Article
Corrections and comments
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Authors and funding
27 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular and molecular actions of general anesthetics to induce anesthesia state and also cellular signaling changes for subsequent potential "long term" effects remain largely elusive. General anesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels. Here we used single-cell RNA-sequencing complemented with whole-cell patch clamp and calcium transient techniques to examine the gene transcriptome and ion channels profiling of sevoflurane and propofol, both commonly used clinically, on the human fetal prefrontal cortex (PFC) mixed cell cultures. Both propofol and sevoflurane at clinically relevant dose/concentration promoted "microgliosis" but only sevoflurane decreased microglia transcriptional similarity. Propofol and sevoflurane each extensively but transiently (<2 h) altered transcriptome profiling across microglia, excitatory neurons, interneurons, astrocytes and oligodendrocyte progenitor cells. Utilizing scRNA-seq as a robust and high-through put tool, our work may provide a comprehensive blueprint for future mechanistic studies of general anesthetics in clinically relevant settings.
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