ArticleNature food2022
A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation.
Article in Nature food, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 200 citations in OpenAlex.
- MuSC cultivation for cultured meat production: recent advances, current limitations, and future directions.Food science of animal resources · 2026Review
- Spatial heterogeneity of lineage-committed muscle stem cell subpopulations challenges bipotency assumptions in Thai-French crossbred cattle.Veterinary world · 2026Article
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- 3D bio-screen printing for high-throughput production of scaffolds for meat alternatives.NPJ science of food · 2026Article
- Generation of multitissue cell-cultivated meat via multidirectional differentiation of stable porcine epiblast stem cells.Nature communications · 2026Article
- Hastening meat production: a review of growth promoters for traditional and cultured meat production.Journal of animal science and technology · 2026Review
- Comparing meat alternatives for a sustainable food system.NPJ science of food · 2026Article
- A small molecule strategy with forskolin and p38 inhibitor for serum-free muscle stem cell expansion.NPJ science of food · 2026Article
- Exploring ethical, sustainable and effective foetal bovine serum alternatives forFrontiers in toxicology · 2026Review
- Cell sources and engineering strategies for cultured meat production: a critical comparative review.Frontiers in nutrition · 2026Review
- Serum withdrawal establishes a stress-dominant entry state during myogenic differentiation.Frontiers in cell and developmental biology · 2026Article
- Growth factors-infused cellulose scaffolds support cost-efficient proliferation and differentiation of bovine stem cells for cultivated meat.Current research in food science · 2026Article
- Fundamentals of Cultivated Meat Technology: Cell Culture and Tissue Engineering (Essentials of Cell Culture, Know-How Transfer from the Red Biotechnology).Advances in biochemical engineering/biotechnology · 2026Review
- Bioprocess Engineering for Cultivated Meat.Advances in biochemical engineering/biotechnology · 2026Review
- Addressing hurdles in cultured meat by exploring reduced myogenesis after bovine myoblast expansion.Communications biology · 2025Article
- Unravelling bovine preadipocyte differentiation and their three-dimensional cultivation for cellular agriculture.NPJ science of food · 2025Article
- High-Throughput Label-Free Continuous Quantification of Muscle Stem Cell Proliferation and Myogenic Differentiation.Stem cell reviews and reports · 2025Article
- Dairy byproducts as sustainable alternatives to FCS in 2D and 3D skeletal muscle cell cultures.Bioresources and bioprocessing · 2025Article
- Serum-free cultured meat production by usingiScience · 2025Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components. Current protocols induce myogenic differentiation in vitro through serum starvation, that is, an abrupt reduction in serum concentration. Here we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation, including IGF1R, TFRC and LPAR1. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Moreover, the serum-free differentiation media supported the fabrication of three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.