Evidence map›Paper›PMID 37118429›Full record

ArticleNature aging2023

Heterochronic parabiosis reprograms the mouse brain transcriptome by shifting aging signatures in multiple cell types.

Methodios Ximerakis, Kristina M Holton, Richard M Giadone, Ceren Ozek, Monika Saxena, Samara Santiago, Xian Adiconis, Danielle Dionne, Lan Nguyen, Kavya M Shah and 10 more

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature aging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
10.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 67 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Stem Cells in Aging and Anti-Aging.Stem cell reviews and reports · 2026
    Review
  7. Research progress on blood therapy for anti-aging.Journal of advanced research · 2026
    Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Parabiosis, Assembloids, Organoids (PAO).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  13. Review
  14. Microvascular Health as a Key Determinant of Organismal Aging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. A Machine-Learning Approach Identifies Rejuvenating Interventions in the Human Brain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 3 institutions in 1 country.

Methodios Ximerakis *Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. methodiosximerakis@gmail.com.ORCID http://orcid.org/0000-0002-2815-7558
Kristina M Holton *Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Richard M GiadoneDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-4523-3062
Ceren OzekDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Monika SaxenaDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Samara SantiagoDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-9502-7104
Xian AdiconisStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Danielle DionneKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Lan NguyenKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Kavya M ShahDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-8722-4095
Jill M GoldsteinDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Caterina GasperiniDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Ioannis A GampierakisDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Scott L LipnickDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Sean K SimmonsStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Sean M BuchananDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Amy J WagersDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Aviv RegevKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-3293-3158
Joshua Z LevinStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-0170-3598
Lee L RubinDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. lee_rubin@harvard.edu.ORCID http://orcid.org/0000-0002-8658-841X
Broad Institute · USHarvard University · USJoslin Diabetes Center · US

Funding

Predoctorial Training in Basic NeuroscienceT32NS007433 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MERINEY, STEPHEN D · 1998 to 2022
$8.0M
TRAINING GRANT IN ACADEMIC ENDOCRINOLOGYT32DK007529 · NIDDK · BRIGHAM AND WOMEN'S HOSPITAL · PI Ursula B. Kaiser, Anand Vaidya · 1986 to 2026
$6.3M
Direct and Indirect Effects of GDF11 in the Aging Central Nervous SystemR01AG072086 · NIA · HARVARD UNIVERSITY · PI RUBIN, LEE L · 2020 to 2024
$3.1M
Identifying and Correcting Dementia-Associated Changes in the Blood-Brain BarrierRF1NS117407 · NINDS · HARVARD UNIVERSITY · PI RUBIN, LEE L · 2020 to 2020
$2.4M
Identifying and Correcting Dementia-Associated Changes in the Blood-Brain BarrierR01NS117407 · NINDS · HARVARD UNIVERSITY · PI RUBIN, LEE L · 2024 to 2024
$592k
Understanding Roles for Protein Homeostasis Machinery in Aging Brain VasculatureF32AG079593 · NIA · HARVARD UNIVERSITY · PI GIADONE, RICHARD · 2022 to 2024
$218k
NIA NIH HHS R01 AG072086NIDDK NIH HHS T32 DK007529NINDS NIH HHS R01 NS117407NINDS NIH HHS RF1 NS117407NINDS NIH HHS T32 NS007433
6 · The paper itself

Abstract

Aging is a complex process involving transcriptomic changes associated with deterioration across multiple tissues and organs, including the brain. Recent studies using heterochronic parabiosis have shown that various aspects of aging-associated decline are modifiable or even reversible. To better understand how this occurs, we performed single-cell transcriptomic profiling of young and old mouse brains after parabiosis. For each cell type, we cataloged alterations in gene expression, molecular pathways, transcriptional networks, ligand-receptor interactions and senescence status. Our analyses identified gene signatures, demonstrating that heterochronic parabiosis regulates several hallmarks of aging in a cell-type-specific manner. Brain endothelial cells were found to be especially malleable to this intervention, exhibiting dynamic transcriptional changes that affect vascular structure and function. These findings suggest new strategies for slowing deterioration and driving regeneration in the aging brain through approaches that do not rely on disease-specific mechanisms or actions of individual circulating factors.

Indexed as

Endothelial CellsTranscriptomeAgingAnimalsBrainMiceParabiosis

Identifiers

PMID37118429
PMCPMC10154248
OpenAlexW4327698219

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.