ArticleNature communications2023
RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Force-Locking DNA Hairpin Probes for High-Throughput and Cumulative Detection of Intercellular Molecular Tensions.Angewandte Chemie (International ed. in English) · 2026Article
- Single Cell Mechanics in Disease Progression.Small science · 2026Review
- Homogeneous Antibody-DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion Proteins.Bioconjugate chemistry · 2026Article
- Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags.Nucleic acids research · 2026Article
- Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies.Nature protocols · 2026Review
- Synthetic aptamer mechanoreceptors enable cell-specific force sensing and temporal control via DNA circuits.Nature communications · 2026Article
- Synthetic mechanoreceptor engineering: From genetic encoding to DNA nanotechnology-based reprogramming.Mechanobiology in medicine · 2025Review
- High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells.Journal of visualized experiments : JoVE · 2025Article
- Near-Infrared Light-Controlled Dynamic Hydrogel for Modulating Mechanosensitive Ion Channels in 3-Dimensional Environment.Biomaterials research · 2025Article
- Nuclease-Resistant L-DNA Tension Probes Enable Long-Term Force Mapping of Single Cells and Cell Consortia.Angewandte Chemie (International ed. in English) · 2024Article
- Mutual antagonism between CD44 and integrins in glioblastoma cell traction and migration.APL bioengineering · 2024Article
- HUHgle: An Interactive Substrate Design Tool for Covalent Protein-ssDNA Labeling Using HUH-Tags.ACS synthetic biology · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Glioblastoma Cells Use an Integrin- and CD44-Mediated Motor-Clutch Mode of Migration in Brain Tissue.Cellular and molecular bioengineering · 2024Article
- Electrochemical DNA-based sensors for measuring cell-generated forces.bioRxiv : the preprint server for biology · 2023Article
- Glioblastoma cells use an integrin- and CD44-mediated motor-clutch mode of migration in brain tissue.bioRxiv : the preprint server for biology · 2023Article
- Optimal cell traction forces in a generalized motor-clutch model.Biophysical journal · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Mechanical forces drive critical cellular processes that are reflected in mechanical phenotypes, or mechanotypes, of cells and their microenvironment. We present here "Rupture And Deliver" Tension Gauge Tethers (RAD-TGTs) in which flow cytometry is used to record the mechanical history of thousands of cells exerting forces on their surroundings via their propensity to rupture immobilized DNA duplex tension probes. We demonstrate that RAD-TGTs recapitulate prior DNA tension probe studies while also yielding a gain of fluorescence in the force-generating cell that is detectable by flow cytometry. Furthermore, the rupture propensity is altered following disruption of the cytoskeleton using drugs or CRISPR-knockout of mechanosensing proteins. Importantly, RAD-TGTs can differentiate distinct mechanotypes among mixed populations of cells. We also establish oligo rupture and delivery can be measured via DNA sequencing. RAD-TGTs provide a facile and powerful assay to enable high-throughput mechanotype profiling, which could find various applications, for example, in combination with CRISPR screens and -omics analysis.
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