Evidence map›Paper›PMID 37115802›Full record

ArticlePloS one2023

TGM2 inhibits the proliferation, migration and tumorigenesis of MDCK cells.

Zhenyu Qiu, Shouqing Guo, Geng Liu, Mengyuan Pei, Yuejiao Liao, Jiamin Wang, Jiayou Zhang, Di Yang, Zilin Qiao, Zhuo Li and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Zhenyu QiuNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Shouqing GuoNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Geng LiuNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Mengyuan PeiNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Yuejiao LiaoNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Jiamin WangNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Jiayou ZhangNational Engineering Technology Research Center of Combined Vaccines, Wuhan, China.
Di YangNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Zilin QiaoNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Zhuo LiNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Zhongren MaNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Zhenbin LiuNorthwest Minzu University, Biomedical Research Center, Gansu Tech Innovation Center of Animal Cell, Lanzhou, China.
Xiaoming YangNational Engineering Technology Research Center of Combined Vaccines, Wuhan, China.
Minzu University of China · CNState Ethnic Affairs Commission · CNChina National Biotechnology (China) · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Madin-Darby canine kidney (MDCK) cells are one of the main cell lines used for influenza vaccine production due to their high virus yield and low mutation resistance. Due to their high tumorigenicity, the safety of vaccines produced from these cells is controversial. TGM2 is a multifunctional protein that plays an important role in the adhesion and migration of cells and is associated with tumor formation. We found that the expression level of TGM2 was significantly up-regulated in low tumorigenic MDCK cells. We first analyzed TGM2-overexpressed and knockout MDCK cells in vitro. Scratch-wound assay and Transwell chamber experiments showed that TGM2 overexpression significantly inhibited the migration and invasion of MDCK cells and significantly reduced their proliferation. TGM2 knockout significantly enhanced cell migration, invasion, and proliferation. The tumorigenesis results in nude mice were consistent with those in vitro. TGM2 knockout significantly enhanced the tumorigenesis rate of MDCK cells in nude mice. We also investigated the effects of TGM2 gene expression on the replication of the H1N1 influenza A virus in MDCK cells. The results showed that TGM2 induced the negative regulation of H1N1 replication. These findings contribute to a comprehensive understanding of the tumor regulation mechanism and biological functions of TGM2.

Indexed as

Influenza A virusInfluenza A Virus, H1N1 SubtypeAnimalsCarcinogenesisCell ProliferationDogsMadin Darby Canine Kidney CellsMiceMice, NudeProtein Glutamine gamma Glutamyltransferase 2Protein Glutamine gamma Glutamyltransferase 2

Identifiers

PMID37115802
PMCPMC10146566
OpenAlexW4367309406

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.