ArticlePloS one2023
Phenotypic screening using waveform analysis of synchronized calcium oscillations in primary cortical cultures.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Diverse Effects of Various Toll-Like Receptor 2 Ligands on Neuronal Activity and Cell Death.Cellular and molecular neurobiology · 2025Article
- Comparative Study on Modulation of Synchronized Neuronal Activity by SK Channels and Na/K-ATPase.International journal of molecular sciences · 2025Article
- Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons.Scientific reports · 2025Article
- Study of the Synchronization and Transmission of Intracellular Signaling Oscillations in Cells Using Bispectral Analysis.Biology · 2024Article
- Regulation of specific abnormal calcium signals in the hippocampal CA1 and primary cortex M1 alleviates the progression of temporal lobe epilepsy.Neural regeneration research · 2024Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
At present, in vitro phenotypic screening methods are widely used for drug discovery. In the field of epilepsy research, measurements of neuronal activities have been utilized for predicting efficacy of anti-epileptic drugs. Fluorescence measurements of calcium oscillations in neurons are commonly used for measurement of neuronal activities, and some anti-epileptic drugs have been evaluated using this assay technique. However, changes in waveforms were not quantified in previous reports. Here, we have developed a high-throughput screening system containing a new analysis method for quantifying waveforms, and our method has successfully enabled simultaneous measurement of calcium oscillations in a 96-well plate. Features of waveforms were extracted automatically and allowed the characterization of some anti-epileptic drugs using principal component analysis. Moreover, we have shown that trajectories in accordance with the concentrations of compounds in principal component analysis plots were unique to the mechanism of anti-epileptic drugs. We believe that an approach that focuses on the features of calcium oscillations will lead to better understanding of the characteristics of existing anti-epileptic drugs and allow to predict the mechanism of action of novel drug candidates.
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