Evidence map›Paper›PMID 37115252›Full record

ArticleApplied microbiology and biotechnology2023

Synthesis of 2'-fucosyllactose from apple pomace-derived xyloglucan oligosaccharides by an α-L-fucosidase from Pedobacter sp. CAU209.

Ran Shi, Shao-Qing Yang, Nan-Nan Wang, Qiao-Juan Yan, Xie-Min Yan, Zheng-Qiang Jiang

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Ran Shi *Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, 100083, Beijing, People's Republic of China.
Shao-Qing Yang *Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, 100083, Beijing, People's Republic of China.
Nan-Nan WangKey Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, 100083, Beijing, People's Republic of China.
Qiao-Juan YanCollege of Engineering, China Agricultural University, Haidian District, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, People's Republic of China.
Xie-Min YanCollege of Engineering, China Agricultural University, Haidian District, No.17 Qinghua Donglu, Haidian District, Beijing, 100083, People's Republic of China.
Zheng-Qiang JiangKey Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, 100083, Beijing, People's Republic of China. zhqjiang@cau.edu.cn.ORCID http://orcid.org/0000-0002-9573-2890
China Agricultural University · CNChinese Academy of Medical Sciences & Peking Union Medical College · CNNanjing University of Finance and Economics · CN

Funding

National Key Research and Development Program 2021YFC2100302
6 · The paper itself

Abstract

2'-Fucosyllactose (2'-FL) is known for its ability to provide various health benefits to infants, such as gut maturation, pathogen resistance, improved immunity, and nervous system development. However, the production of 2'-FL using α-L-fucosidases is hindered by the lack of low-cost natural fucosyl donors and high-efficiency α-L-fucosidases. In this work, a recombinant xyloglucanase from Rhizomucor miehei (RmXEG12A) was applied to produce xyloglucan-oligosaccharide (XyG-oligos) from apple pomace. Then, an α-L-fucosidase gene (PbFucB) was screened from the genomic DNA of Pedobacter sp. CAU209 and expressed in Escherichia coli. The capability of purified PbFucB to catalyze XyG-oligos and lactose to synthesize 2'-FL was further evaluated. The deduced amino acid sequence of PbFucB shared the highest identity (38.4%) with that of other reported α-L-fucosidases. PbFucB showed the highest activity at pH 5.5 and 35 °C. It catalyzed the hydrolysis of 4-nitrophenyl-α-L-fucopyranoside (pNP-Fuc, 20.3 U mg

Indexed as

MalusPedobacteralpha-L-FucosidaseGlucansHumansInfantLactoseOligosaccharidesRhizomucorTrisaccharidesXylans2'-fucosyllactosealpha-L-FucosidaseGlucansLactoseOligosaccharidesTrisaccharidesXylansxyloglucan2′-FucosyllactoseApple pomacePedobacter sp.Xyloglucan-oligosaccharideα-L-fucosidase

Identifiers

PMID37115252
OpenAlexW4367298051

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.