Evidence map›Paper›PMID 37113725›Full record

ArticleEcancermedicalscience2023

Improvement of diagnosis in children with Burkitt lymphoma in Kenya: feasibility study for the implementation of fluorescence

Gail H Vance, Teresa Lotodo, Nicholas Kigen, Ryan Stohler, Haki Choi, Festus Njuguna, Ann M Moormann, Erastus Kirwa, Sandra Langat, Patrick Loehrer and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ecancermedicalscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 2 countries.

Gail H VanceDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Teresa LotodoDepartment of Pathology, Moi University, Eldoret, Kenya.
Nicholas KigenAMPATH Reference Laboratory, Eldoret, Kenya.
Ryan StohlerDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Haki ChoiDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Festus NjugunaDepartment of Child Health and Pediatrics, Moi University, Eldoret, Kenya.
Ann M MoormannDepartment of Medicine, University of Massachusetts Chan Medical School, Worcester, MA 01655, USA.
Erastus KirwaKenya Medical Research Institute (KEMRI), Kisumu, Kenya.
Sandra LangatAMPATH Pediatric Oncology, Eldoret, Kenya.
Patrick LoehrerDepartment of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Terry VikDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Indiana University School of MedicineAMPATH · KEIndiana University – Purdue University Indianapolis · USMoi University · KEKenya Medical Research Institute · KEUniversity of Massachusetts Chan Medical School · US

Funding

Tumor Microenvironment and Metastasis ProgramP30CA082709 · NCI · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI Jun Wan · 1999 to 2026
$59.3M
NCI NIH HHS P30 CA082709
6 · The paper itself

Abstract

Background: Indiana University (IU) initiated fluorescence in situ hybridisation (FISH) methodology for Burkitt Lymphoma (BL) to advance the accuracy and speed of diagnosis in the AMPATH Reference Laboratory at Moi Teaching and Referral Hospital (MTRH) in Eldoret, Kenya. Standard diagnostic testing for BL at MTRH includes morphology of the biopsy specimen or aspirate and limited immunohistochemistry panels. Methods: Tumour specimens from 19 children enrolled from 2016 to 2018 in a prospective study to improve the diagnosis and staging of children with suspected BL were evaluated. Touch preps from biopsy specimens or smears from fine needle aspiration were collected, stained with Giemsa and/or H&E and reviewed by pathologists to render a provisional diagnosis. Unstained slides were stored and later processed for FISH. Duplicate slides were split between two laboratories for analysis. Flow cytometry results were available for all specimens. Results from the newly established FISH laboratory in Eldoret, Kenya were cross-validated in Indianapolis, Indiana. Results: Concordance studies found 18 of 19 (95%) of specimens studied yielded analysable FISH results for one or both probe sets ( Conclusion: FISH testing was established, and a pilot study performed, to assess the feasibility of FISH as a diagnostic tool for the determination of BL in a Kenyan paediatric population. This study supports FISH in limited resource settings to improve the accuracy and speed of diagnosis of BL in Africa.

Indexed as

Burkitt lymphomaFISHfluorescence in situ hybridisationMYC/IGH translocationMYC rearrangement

Identifiers

PMID37113725
PMCPMC10129372
OpenAlexW4319599689

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.