ArticleViruses2023
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Lumpy Skin Disease Virus (LSDV) recently emerged in Egypt: molecular detection, and assessment of the related hematobiochemical, and risk factors.BMC veterinary research · 2026Article
- Article
- Comprehensive Evaluation of the Immune Response of Angus Cattle to Live Attenuated and Inactivated Goatpox Virus Vaccines.Animals : an open access journal from MDPI · 2025Article
- Molecular detection and phylogenetic analysis of lumpy skin disease virus in Yunnan Province, China from 2019 to 2023.Veterinary research communications · 2025Article
- Article
- Development of a Multi-Locus Real-Time PCR with a High-Resolution Melting Assay to Differentiate Wild-Type, Asian Recombinant, and Vaccine Strains of Lumpy Skin Disease Virus.Veterinary sciences · 2025Article
- Monitoring of genetic alterations of lumpy skin disease virus in cattle after vaccination in Thailand.Journal of animal science and technology · 2025Article
- Article
- Genome sequence characterization of the unique recombinant vaccine-like lumpy skin disease virus strain Kurgan/2018.Archives of virology · 2024Article
- Development of a TaqMan polymerase chain reaction detection method for the precise identification and quantification of an attenuatedFrontiers in veterinary science · 2024Article
- Polarization- and Chaos-Game-Based Fingerprinting of Molecular Targets of Listeria Monocytogenes Vaccine and Fully Virulent Strains.Current issues in molecular biology · 2023Article
- Complete genome reconstruction of the global and European regional dispersal history of the lumpy skin disease virus.Journal of virology · 2023Article
- Development and application of a real-time polymerase chain reaction assay to detect lumpy skin disease virus belonging to the Kenyan sheep and goat pox group.BMC research notes · 2023Article
- Review
- Lumpy skin disease: history, current understanding and research gaps in the context of recent geographic expansion.Frontiers in microbiology · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current epidemic in Asia, driven by LSDV recombinants, poses difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of differentiating Neethling-based vaccine strains from classical and recombinant wild-type strains that are currently circulating in Asia. The DIVA potential of this new assay, seen in the in silico evaluation, was confirmed on samples from LSDV infected and vaccinated animals and on isolates of LSDV recombinants (n = 12), vaccine (n = 5), and classic wild-type strains (n = 6). No cross-reactivity or a-specificity with other capripox viruses was observed under field conditions in non-capripox viral stocks and negative animals. The high analytical sensitivity is translated into a high diagnostic specificity as more than 70 samples were all correctly detected with Ct values very similar to those of a published first-line pan capripox real-time PCR. Finally, the low inter- and intra-run variability observed shows that the new DIVA PCR is very robust which facilitates its implementation in the lab. All validation parameters that are mentioned above indicate the potential of the newly developed test as a promising diagnostic tool which could help to control the current LSDV epidemic in Asia.
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