Evidence map›Paper›PMID 37112313›Full record

ArticleSensors (Basel, Switzerland)2023

Sensing of Catecholamine in Human Urine Using a Simple Colorimetric Assay Based on Direct Melanochrome and Indolequinone Formation.

Mariagrazia Lettieri, Michele Spinelli, Laura Caponi, Simona Scarano, Pasquale Palladino, Angela Amoresano, Maria Minunni

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Mariagrazia LettieriDepartment of Chemistry 'Ugo Schiff', University of Florence, 50019 Sesto Fiorentino, Italy.ORCID 0000-0001-6945-7576
Michele SpinelliDepartment of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.ORCID 0000-0001-8252-1587
Laura CaponiLaboratory of Clinical Pathology, University Hospital of Pisa, 56126 Pisa, Italy.ORCID 0000-0001-9597-9749
Simona ScaranoDepartment of Chemistry 'Ugo Schiff', University of Florence, 50019 Sesto Fiorentino, Italy.ORCID 0000-0002-8050-1715
Pasquale PalladinoDepartment of Chemistry 'Ugo Schiff', University of Florence, 50019 Sesto Fiorentino, Italy.ORCID 0000-0002-3869-5085
Angela AmoresanoDepartment of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.
Maria MinunniDepartment of Chemistry 'Ugo Schiff', University of Florence, 50019 Sesto Fiorentino, Italy.ORCID 0000-0003-0906-0862
University of Florence · ITAzienda Ospedaliera Universitaria Pisana · ITIstituto Nazionale Biostrutture e Biosistemi · ITUniversity of Naples Federico II · IT

Funding

Ministry of University and Research (MUR) Dipartimenti di Eccellenza
6 · The paper itself

Abstract

We used the first enzyme-free synthesis and stabilization of soluble melanochrome (MC) and 5,6-indolequinone (IQ) derived from levodopa (LD), dopamine (DA), and norepinephrine (NE) oxidation to develop a simple colorimetric assay for catecholamine detection in human urine, also elucidating the time-dependent formation and molecular weight of MC and IQ using UV-Vis spectroscopy and mass spectrometry. The quantitative detection of LD and DA was achieved in human urine using MC as a selective colorimetric reporter to demonstrate the potential assay applicability in a matrix of interest in therapeutic drug monitoring (TDM) and in clinical chemistry. The assay showed a linear dynamic range between 5.0 mg L

Indexed as

CatecholaminesIndolequinonesColorimetryDopamineHumansLevodopaReproducibility of ResultsCatecholaminesDopamineIndolequinonesLevodopacatecholamines oxidationcolorimetric assaydopamineindolequinonelevodopamelanochromenorepinephrineParkinson’s diseasetherapeutic drug monitoringurine analysis

Identifiers

PMID37112313
PMCPMC10146333
OpenAlexW4365515123

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.