Evidence map›Paper›PMID 37111797›Full record

ArticlePharmaceutics2023

Activation of Insulin Gene Expression via Transfection of a CRISPR/dCas9a System Using Magnetic Peptide-Imprinted Nanoparticles.

Mei-Hwa Lee, James L Thomas, Chien-Yu Lin, Yi-Chen Ethan Li, Hung-Yin Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Mei-Hwa LeeDepartment of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
James L ThomasDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.
Chien-Yu LinDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Yi-Chen Ethan LiDepartment of Chemical Engineering, Feng Chia University, Taichung 40724, Taiwan.
Hung-Yin LinDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
National University of Kaohsiung · TWFeng Chia University · TWI-Shou University · TWUniversity of New Mexico · US

Funding

Ministry of Science and Technology of ROC MOST 109-2221-E-035-007-MY3Ministry of Science and Technology of ROC MOST 109-2314-B-390-001-MY3Ministry of Science and Technology of ROC MOST 110-2221-E-390-003-MY3
6 · The paper itself

Abstract

A CRISPRa transcription activation system was used to upregulate insulin expression in HEK293T cells. To increase the delivery of the targeted CRISPR/dCas9a, magnetic chitosan nanoparticles, imprinted with a peptide from the Cas9 protein, were developed, characterized, and then bound to dCas9a that was complexed with a guide RNA (gRNA). The adsorption of dCas9 proteins conjugated with activators (SunTag, VPR, and p300) to the nanoparticles was monitored using both ELISA kits and Cas9 staining. Finally, the nanoparticles were used to deliver dCas9a that was complexed with a synthetic gRNA into HEK293T cells to activate their insulin gene expression. Delivery and gene expression were examined using quantitative real-time polymerase chain reaction (qRT-PCR) and staining of insulin. Finally, the long-term release of insulin and the cellular pathway related to stimulation by glucose were also investigated.

Indexed as

CRISPR/dCas9agene activationgene expressioninsulinmolecular imprinting

Identifiers

PMID37111797
PMCPMC10144049
OpenAlexW4366772228

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.