ArticleMicroorganisms2023
Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells.
Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose.Microbial cell factories · 2026Article
- Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.Folia microbiologica · 2025Article
- Simulation Study of Low-Dose 4D-STEM Phase Contrast Techniques at the Nanoscale in SEM.Nanomaterials (Basel, Switzerland) · 2025Article
- 'Candidatus Pseudomonas auctus' sp. nov. JDE115 isolated from nodules on soybean (Glycines max).PloS one · 2025Article
- Cultivation driven transcriptomic changes in the wild-type and mutant strains ofComputational and structural biotechnology journal · 2024Article
- Dedicated Protocol for Ultrastructural Analysis of Farmed Rainbow Trout (Methods and protocols · 2024Article
- Transdisciplinary approaches for the study of cyanobacteria and cyanotoxins.Current research in microbial sciences · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.
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