ArticleInternational journal of molecular sciences2023
Extracellular Vesicles Secreted by Pre-Hatching Bovine Embryos Produced In Vitro and In Vivo Alter the Expression of IFNtau-Stimulated Genes in Bovine Endometrial Cells.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Embryo-derived extracellular vesicles and their potential as biomarkers for embryo quality during IVF: a systematic review.Human reproduction update · 2026Pooled it
- Coding and Noncoding Uterine Small Extracellular Vesicle Content Differs in the Early Stages of Pregnancies Produced by Artificial Insemination and In Vitro Fertilization in Cattle.Molecular reproduction and development · 2026Article
- Comparative profiling of extracellular vesicles and miRNA cargo from in vivo- and in vitro-derived bovine embryos during blastulation and hatching.Journal of animal science and biotechnology · 2026Article
- The Impact of Melatonin on Cellular Dynamics and Gene Expression of Bovine Embryos Cultured Under Low and High Oxygen Tension.Molecular reproduction and development · 2026Article
- Extracellular Vesicles in Implantation: Cross-Talk Between the Embryo and Endometrium.Advances in anatomy, embryology, and cell biology · 2026Review
- LPS-Stressed Bovine Endometrial Cells upon Morulae in a Transwell Model of Embryo-Maternal Talk.Animals : an open access journal from MDPI · 2025Article
- Deciphering the dialogue between the bovine blastocyst and the uterus: embryo-induced alterations in extracellular vesicle protein content from an ex vivo model and the in vivo environment.Journal of animal science and biotechnology · 2025Article
- Review
- Deciphering embryo-maternal cross-talk: advanced in vitro approaches.Animal reproduction · 2025Article
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- Biogenesis of Extracellular Vesicles (EVs) and the Potential Use of Embryo-Derived EVs in Medically Assisted Reproduction.International journal of molecular sciences · 2024Review
- Extracellular Vesicles: Biology and Therapeutic Applications.International journal of molecular sciences · 2024Article
- Exploring the potential of in vitro extracellular vesicle generation in reproductive biology.Journal of extracellular biology · 2024Review
- DNA Content in Embryonic Extracellular Vesicles Is Independent of the Apoptotic Rate in Bovine Embryos Produced In Vitro.Animals : an open access journal from MDPI · 2024Article
- Effect of IGFBP-4 during In Vitro Maturation on Developmental Competence of Bovine Cumulus Oocyte Complexes.Animals : an open access journal from MDPI · 2024Article
- Extracellular vesicles: emerging paradigms in bovine embryo-maternal communication.Animal reproduction · 2024Review
- Extracellular Vesicles Derived from Selenium-Deficient MAC-T Cells Aggravated Inflammation and Apoptosis by Triggering the Endoplasmic Reticulum (ER) Stress/PI3K-AKT-mTOR Pathway in Bovine Mammary Epithelial Cells.Antioxidants (Basel, Switzerland) · 2023Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
The embryo-maternal interaction occurs during the early stages of embryo development and is essential for the implantation and full-term development of the embryo. In bovines, the secretion of interferon Tau (IFNT) during elongation is the main signal for pregnancy recognition, but its expression starts around the blastocyst stage. Embryos release extracellular vesicles (EVs) as an alternative mechanism of embryo-maternal communication. The aim of the study was to determine whether EVs secreted by bovine embryos during blastulation (D5-D7) could induce transcriptomic modifications, activating IFNT signaling in endometrial cells. Additionally, it aims to assess whether the EVs secreted by embryos produced in vivo (EVs-IVV) or in vitro (EVs-IVP) have different effects on the transcriptomic profiles of the endometrial cells. In vitro- and in vivo-produced bovine morulae were selected and individually cultured for 48 h to collect embryonic EVs (E-EVs) secreted during blastulation. E-EVs stained with PKH67 were added to in vitro-cultured bovine endometrial cells to assess EV internalization. The effect of EVs on the transcriptomic profile of endometrial cells was determined by RNA sequencing. EVs from both types of embryos induced several classical and non-classical IFNT-stimulated genes (ISGs) and other pathways related to endometrial function in epithelial endometrial cells. Higher numbers of differentially expressed genes (3552) were induced by EVs released by IVP embryos compared to EVs from IVV (1838). Gene ontology analysis showed that EVs-IVP/IVV induced the upregulation of the extracellular exosome pathway, the cellular response to stimulus, and the protein modification processes. This work provides evidence regarding the effect of embryo origin (in vivo or in vitro) on the early embryo-maternal interaction mediated by extracellular vesicles.
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Registered trials
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