ArticleInternational journal of molecular sciences2023
A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Raman spectral unmixing for quantitative analysis of multicomponent amino acid mixtures.Analytical and bioanalytical chemistry · 2026Article
- Performance comparison of downstream unit operations and process chains for the purification of amino acids for food, feed and pharma applications.Biotechnology reports (Amsterdam, Netherlands) · 2026Review
- Targeted Chiral Metabolomics of D-Amino Acids: Their Emerging Role as Potential Biomarkers in Neurological Diseases with a Focus on Their Liquid Chromatography-Mass Spectrometry Analysis upon Chiral Derivatization.International journal of molecular sciences · 2024Review
- Development of an ultra-sensitive laser stimulated fluorescence system for simultaneous detection of amino acids.RSC advances · 2024Article
- Editorial of Special Issue "Current Trends in Chemistry Towards Biology".International journal of molecular sciences · 2024Article
- UHPLC-QQQ-MS/MS method for the simultaneous quantification of 18 amino acids in various meats.Frontiers in nutrition · 2024Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.
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Registered trials
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