Evidence map›Paper›PMID 37108239›Full record

ArticleInternational journal of molecular sciences2023

3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model.

Yu-Ying Lin, Che-Yung Kuan, Chia-Tien Chang, Ming-Hsi Chuang, Wan-Sin Syu, Kai-Ling Zhang, Chia-Hsin Lee, Po-Cheng Lin, Guo-Chung Dong, Feng-Huei Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Yu-Ying LinPh.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Che-Yung KuanInstitute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County 35053, Taiwan.
Chia-Tien ChangInstitute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County 35053, Taiwan.
Ming-Hsi ChuangCollege of Management, Chung Hwa University, Hsinchu 30012, Taiwan.
Wan-Sin SyuGwo Xi Stem Cell Applied Technology, Hsinchu 30261, Taiwan.
Kai-Ling ZhangGwo Xi Stem Cell Applied Technology, Hsinchu 30261, Taiwan.
Chia-Hsin LeeGwo Xi Stem Cell Applied Technology, Hsinchu 30261, Taiwan.
Po-Cheng LinGwo Xi Stem Cell Applied Technology, Hsinchu 30261, Taiwan.ORCID 0000-0002-3150-2471
Guo-Chung DongPh.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Feng-Huei LinPh.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 40227, Taiwan.ORCID 0000-0002-2994-6671
National Chung Hsing University · TWStem Cell Technology (Taiwan) · TWNational Health Research Institutes · TWChung Hua University · TWNational Yang Ming Chiao Tung University · TW

Funding

National Health Research Institutes BN-110-PP-01, BN-110-GP-09 and BN-111-PP-01
6 · The paper itself

Abstract

Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) scaffolds were prepared for human adipose-derived stem cell (hADSC) proliferation with a homemade functionally closed process bioreactor system; the feasibility of cultured hADSC spheres in heterologous stem cell therapy for OA treatment was then evaluated. hADSC spheres were collected from Ca-Ag scaffolds by removing calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation. In this study, 2D-cultured individual hADSCs or hADSC spheres were evaluated for treatment efficacy in a monosodium iodoacetate (MIA)-induced OA rat model. The results of gait analysis and histological sectioning showed that hADSC spheres were more effective at relieving arthritis degeneration. The results of serological and blood element analyses of hADSC-treated rats indicated that the hADSC spheres were a safe treatment in vivo. This study demonstrates that hADSC spheres are a promising treatment for OA and can be applied to other stem cell therapies or regenerative medical treatments.

Indexed as

Mesenchymal Stem CellsOsteoarthritisAdipocytesAlginatesAnimalsCalciumDisease Models, AnimalHumansRatsAlginatesCalciumalginatecell sphereshuman adipose-derived stem cellsmonosodium iodoacetateosteoarthritis

Identifiers

PMID37108239
PMCPMC10138691
OpenAlexW4367320506

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.