ArticleGenes2023
Metagenomics Provides a Deeper Assessment of the Diversity of Bacterial Communities in Polar Soils Than Metabarcoding.
Article in Genes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Molecular markers for the study of arbuscular mycorrhizal fungi.Archives of microbiology · 2026Review
- Impacts of drought and manure fertilization on soil and radish resistomes.Scientific reports · 2026Article
- Consistent microbial insights across sequencing methods in soil studies: the role of reference taxonomies.mSystems · 2025Article
- Metagenomics reveals contrasted responses of microbial communities to wheat straw amendment in cropland and grassland soils.Scientific reports · 2025Article
- Assessment of Soil Health Through Metagenomic Analysis of Bacterial Diversity in Russian Black Soil.Microorganisms · 2025Article
- Bacterial Diversity, Metabolic Profiling, and Application Potential of Antarctic Soil Metagenomes.Current issues in molecular biology · 2024Article
- Diverse patterns of correspondence between protist metabarcodes and protist metagenome-assembled genomes.PloS one · 2024Article
- Multi-omics for studying and understanding polar life.Nature communications · 2023Review
- Metagenomic Analysis of Antarctic Biocrusts Unveils a Rich Range of Cold-Shock Proteins.Microorganisms · 2023Article
- Editorial: Polar Genomics in a Changing World.Genes · 2023Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diversity of soil bacteria was analyzed via metabarcoding and metagenomic approaches using DNA samples isolated from the biocrusts of 12 different Arctic and Antarctic sites. For the metabarcoding approach, the V3-4 region of the 16S rRNA was targeted. Our results showed that nearly all operational taxonomic units (OTUs = taxa) found in metabarcoding analyses were recovered in metagenomic analyses. In contrast, metagenomics identified a large number of additional OTUs absent in metabarcoding analyses. In addition, we found huge differences in the abundance of OTUs between the two methods. The reasons for these differences seem to be (1) the higher sequencing depth in metagenomics studies, which allows the detection of low-abundance community members in metagenomics, and (2) bias of primer pairs used to amplify the targeted sequence in metabarcoding, which can change the community composition dramatically even at the lower taxonomic levels. We strongly recommend using only metagenomic approaches when establishing the taxonomic profiles of whole biological communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.