Evidence map›Paper›PMID 37106219›Full record

ArticleDocumenta ophthalmologica. Advances in ophthalmology2023

Assessment of the uniform field electroretinogram for mouse retinal ganglion cell functional analysis.

Pamela S Lagali, Ushananthini Shanmugalingam, Adam N Baker, Natalie Mezey, Patrice D Smith, Stuart G Coupland, Catherine Tsilfidis

Abstract read
PubMed Publisher
In one paragraph

Article in Documenta ophthalmologica. Advances in ophthalmology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Pamela S LagaliNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada.ORCID 0000-0003-4064-5586
Ushananthini ShanmugalingamDepartment of Neuroscience, Carleton University, Ottawa, ON, Canada.ORCID 0000-0002-1252-8043
Adam N BakerNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada.
Natalie MezeyDepartment of Neuroscience, Carleton University, Ottawa, ON, Canada.ORCID 0000-0001-9596-3640
Patrice D SmithDepartment of Neuroscience, Carleton University, Ottawa, ON, Canada.ORCID 0000-0002-0731-2803
Stuart G CouplandUniversity of Ottawa Eye Institute, The Ottawa Hospital, Ottawa, ON, K1H 8L6, Canada.ORCID 0000-0002-3323-7915
Catherine TsilfidisNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada. ctsilfidis@ohri.ca.ORCID 0000-0002-5206-7384
University of Ottawa · CACarleton University · CA

Funding

Institute of Neurosciences, Mental Health and Addiction 142411
6 · The paper itself

Abstract

purposeThe uniform field electroretinogram (UF-ERG) has been suggested as an alternative to the pattern electroretinogram (PERG) for non-invasive assessment of retinal ganglion cell (RGC) function in primates. We evaluated the validity of the UF-ERG to assess mouse RGC activity in vivo.

methodsUnilateral optic nerve crush (ONC) was performed on adult C57BL/6J mice. Contralateral eyes with uncrushed optic nerves and eyes from surgically naive mice served as experimental controls. Electrophysiological visual assessment was performed at 12 weeks post-ONC. Flash-mediated visual-evoked cortical potentials (VEPs) were measured to confirm the robustness of the ONC procedure. Full-field flash ERGs were used to interrogate photoreceptor and retinal bipolar cell function. RGC function was assessed with pattern ERGs. Summed onset and offset UF-ERG responses to alternating dark and light uniform field flash stimuli of different intensities and wavelengths were recorded from ONC and control eyes, and relative differences were compared to the PERG results. Following electrophysiological analysis, RGC loss was monitored by immunohistochemical staining of the RGC marker protein, RBPMS, in post-mortem retinal tissues.

resultsONC dramatically impacts RGC integrity and optic nerve function, demonstrated by reduced RGC counts and near complete elimination of VEPs. ONC did not affect scotopic ERG a-wave and b-wave amplitudes, while PERG amplitudes of eyes subjected to ONC were reduced by approximately 50% compared to controls. Summation of ON and OFF UF-ERG responses did not reveal statistically significant differences between ONC and control eyes, regardless of visual stimulus.

conclusionsPERG responses are markedly impaired upon ONC, while UF-ERG responses are not significantly affected by surgical trauma to RGC axons in mice. The more closely related pattern and uniform field ERGs recorded in primates suggests species-specific differences in RGC features or subpopulations corresponding to PERG and UF-ERG response generators, limiting the utility of the UF-ERG for mouse RGC functional analysis.

Indexed as

ElectroretinographyRetinal Ganglion CellsAnimalsDisease Models, AnimalMiceMice, Inbred C57BLOptic NerveRetinaMouseOptic nerve crushPattern electroretinogramRetinal ganglion cellsUniform field electroretinogramVisual-evoked potentials

Identifiers

PMID37106219
OpenAlexW2916009918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.