Evidence map›Paper›PMID 37106202›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Vitro Generation of Human Tolerogenic Monocyte-Derived Dendritic Cells.

Catharien M U Hilkens, Julie Diboll, Fiona Cooke, Amy E Anderson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Catharien M U HilkensTranslational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK. catharien.hilkens@newcastle.ac.uk.
Julie DibollTranslational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.
Fiona CookeTranslational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.
Amy E AndersonTranslational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human monocyte-derived dendritic cells (moDC) are commonly used as a research tool to investigate interactions between antigen-presenting cells and T cells. Generation of these cells involves the isolation of CD14 positive monocytes from peripheral blood and their in vitro differentiation into immature moDC by the cytokines GM-CSF and IL-4. Their functional characteristics can then be manipulated by maturing these cells with a cocktail of agents, which can be tailored to induce either immune activating or tolerogenic properties. Here, we describe a protocol for the generation of moDC with stable tolerogenic function, referred to as tolerogenic dendritic cells. These cells have been developed as an immunotherapeutic tool for the treatment of autoimmune disease but have also proven useful to dissect mechanisms of T cell tolerance induction in vitro.

Indexed as

Dendritic CellsMonocytesCell DifferentiationCells, CulturedCytokinesHumansT-LymphocytesCytokines1,25-dihydroxy-vitamin D3Dendritic cellsDexamethasoneGranulocyte-macrophage colony stimulating factorHumanInterleukin-4MonocytesPeripheral blood mononuclear cellsTolerogenic dendritic cellsToll-like receptor 4 ligand

Identifiers

PMID37106202

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.