Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes.

Nagaja Capitani, Chiara Cassioli, Keerthana Ravichandran, Cosima T Baldari

Open access · hybridAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

6.8field-weighted citation impact, top 4% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles.Proceedings of the National Academy of Sciences of the United States of America · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Nagaja CapitaniDepartment of Life Sciences, University of Siena, Siena, Italy. capitani2@unisi.it.

Chiara CassioliDepartment of Life Sciences, University of Siena, Siena, Italy.

Keerthana RavichandranCellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Homburg, Germany.

Cosima T BaldariDepartment of Life Sciences, University of Siena, Siena, Italy.

University of Siena · ITSaarland University · DE

Funding

European Research Council 951329

6 · The paper itself

Abstract

The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.

Indexed as

ProteinsT-Lymphocytes, CytotoxicCytoplasmic GranulesProteinsConfocal microscopyCytotoxic granulesGranzyme BIntracellular traffickingLytic granule biogenesisRUSH systemSpinning disk microscopy

Identifiers

PMID37106198

DOI10.1007/978-1-0716-3135-5_27

PMCPMC7614623

OpenAlexW4367276783

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.