Evidence map›Paper›PMID 37106196›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Isolation of the B Cell Immune Synapse for Proteomic Analysis.

Diogo M Cunha, Sara Hernández-Pérez, Pieta K Mattila

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
5.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Diogo M CunhaInstitute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland.
Sara Hernández-PérezInstitute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland.
Pieta K MattilaInstitute of Biomedicine, MediCity Research Laboratories, and InFLAMES Research Flagship, University of Turku, Turku, Finland. pieta.mattila@utu.fi.
Åbo Akademi University · FIUniversity of Turku · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.

Indexed as

ProteomicsSynapsesAnimalsB-LymphocytesMass SpectrometryMiceProteinsProteinsB cellsCell adhesionImmunological synapse immune synapseMass spectrometryProteomics

Identifiers

PMID37106196
OpenAlexW4367281547

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.