Evidence map›Paper›PMID 37106195›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers.

Hannah M Stephens, Evan Kirkpatrick, Robert J Mallis, Ellis L Reinherz, Matthew J Lang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.0field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Hannah M StephensDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.
Evan KirkpatrickDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.
Robert J MallisLaboratory of Immunobiology and Department of Medical Oncology, Dana-Farber Cancer Institute, and Department of Dermatology, Harvard Medical School, Boston, MA, USA.
Ellis L ReinherzLaboratory of Immunobiology and Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, USA.
Matthew J LangDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA. matt.lang@vanderbilt.edu.
Vanderbilt University · USDana-Farber Cancer Institute · USHarvard University · US

Funding

Protein Production CoreP01AI143565 · NIAID · DANA-FARBER CANCER INST · PI ELLIS L REINHERZ · 2020 to 2026
$19.9M
Understanding the structural basis of T cell receptor (TCR) and preTCR mechanosensing: single molecule, NMR and molecular dynamics studiesR01AI136301 · NIAID · VANDERBILT UNIVERSITY · PI LANG, MATTHEW J. · 2018 to 2022
$3.9M
NIAID NIH HHS P01 AI143565NIAID NIH HHS R01 AI136301
6 · The paper itself

Abstract

αβ T cells are mechanosensors that leverage bioforces during immune surveillance for highly sensitive and specific antigen discrimination. Single-molecule studies are used to profile the initial TCRαβ-pMHC binding event, and various biophysical parameters can be identified. Isolating purified TCRαβ and pMHC molecules on a coverslip allows for direct measurements of the kinetics and conformational changes in the system and removes cellular components along the load pathway that may interfere with or mask subtle changes. Optical tweezers provide high resolution position and force information that map the bonding profile, including catch bond, and the ability to measure distinct conformational changes driven by forces. The present method describes the single-molecule optical tweezers assay setup, considerations, and execution. This model can be used for various TCR-pMHC pairs or expanded to measure a wide variety of receptor-ligand interactions operative in multiple biological systems.

Indexed as

Optical TweezersT-LymphocytesAntigensProtein BindingReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, alpha-betaAntigensReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, alpha-betaCatch bondMechanosensorOptical tweezersSingle moleculeT cellT-cell activationT-cell receptor

Identifiers

PMID37106195
PMCPMC11654165
OpenAlexW4367281720

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.