ArticleMethods in molecular biology (Clifton, N.J.)2023
Functionalized Lipid Droplets and Microfluidics Approach to Study Immune Cell Polarity In Vitro.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
4 authors.
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Abstract
The study of lymphocyte polarization upon antigen encounter typically relies on the random pairing between the cells of interest and a stimulating particle (micro bead) that mimics only some of the properties of the antigen-presenting cells. Here, we show how to build and use a microfluidic chip that allows to multiplex and synchronize the encounter between a lymphocyte and an antigen-presenting object: a functionalized oil-in-water droplet. We also explain how to fabricate and functionalize lipid droplets, an antigen-presenting tool that is, at the same time, deformable, fluid, and spherical.
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Registered trials
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