Evidence map›Paper›PMID 37106190›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Leveraging DNA Origami to Study Phagocytosis.

Wyatt D Miller, Nadja Kern, Shawn M Douglas, Meghan A Morrissey

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wyatt D MillerDepartment of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA.
Nadja KernDepartment of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.
Shawn M DouglasDepartment of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.
Meghan A MorrisseyDepartment of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA. morrissey@ucsb.edu.

Funding

Signal Integration during PhagocytosisR35GM146935 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Meghan A Morrissey · 2022 to 2026
$1.9M
Probing mesoscale receptor organization in T cell signaling with DNA origamiR21AI178200 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DOUGLAS, SHAWN M · 2023 to 2024
$444k
NIAID NIH HHS R21 AI178200NIGMS NIH HHS R35 GM146935
6 · The paper itself

Abstract

Many plasma membrane receptors and ligands form nanoscale clusters on the plasma membrane surface. However, methods for directly and precisely manipulating nanoscale protein localization are limited, making understanding the effects of this clustering difficult. DNA origami allows precise control over nanoscale protein localization with high fidelity and adaptability. Here, we describe how we have used this technique to study how nanoscale protein clustering affects phagocytosis. We provide protocols for conjugating DNA origami structures to supported lipid bilayer-coated beads to assay phagocytosis and planar glass coverslips for TIRF microscopy. The core aspects of this protocol can be translated to study other immune signaling pathways and should enable the implementation of previously inaccessible investigations.

Indexed as

DNAPhagocytosisCell MembraneLipid BilayersSignal TransductionDNALipid BilayersDNA origamiFc ReceptorIgGMacrophagePhagocytosisSignaling

Identifiers

PMID37106190

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.