Evidence map›Paper›PMID 37104024›Full record

ArticleMethods and protocols2023

A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable

M Daben J Libardo, Eberhard Durr, Lorraine D Hernandez

Abstract read
In one paragraph

Article in Methods and protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Exploring the Proteomic Landscape and Immunomodulatory Functions ofJournal of microbiology and biotechnology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

M Daben J LibardoDiscovery Biology, Infectious Diseases & Vaccines, Merck & Co., Inc., West Point, PA 19486, USA.ORCID 0000-0003-0037-7812
Eberhard DurrDiscovery Biology, Infectious Diseases & Vaccines, Merck & Co., Inc., West Point, PA 19486, USA.ORCID 0000-0003-0601-1117
Lorraine D HernandezDiscovery Biology, Infectious Diseases & Vaccines, Merck & Co., Inc., West Point, PA 19486, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Outer membrane vesicles (OMVs) are lipid structures containing various biomolecules in their native environment and are spontaneously shed by gram-negative bacteria. OMVs perform several biological functions critical to both bacterial physiology and pathogenicity. Scientific research on OMV function and biogenesis requires a standardized and robust method of isolating these vesicles from bacterial cultures that reliably provide high-purity OMVs. Herein, we describe an optimized protocol to isolate OMVs from overnight cultures of three different strains of nontypeable

Indexed as

isolation protocolnontypeable Haemophilus influenzaeouter membrane vesicles

Identifiers

PMID37104024
PMCPMC10143165

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.