Evidence map›Paper›PMID 37103112›Full record

ArticleIn vivo (Athens, Greece)

Blueberry Stem Extract Prevents Lacrimal Hyposecretion in Non-obese Diabetic Mice

Kenjirou Ogawa, Yuta Ohno, Akane Tagashira, Karin Urata, Keitaro Satoh, Naruki Fujimoto, Hiroko Sonoda, Masahiro Ikeda, Toshiyuki Matsuzaki, Kazuo Nishiyama and 3 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Kenjirou Ogawa *Institute for Tenure Track Promotion, University of Miyazaki, Miyazaki, Japan; ogawa.kenjirou.u2@cc.miyazaki-u.ac.jp.
Yuta Ohno *Department of Pharmacology, Asahi University School of Dentistry, Mizuho, Japan.
Akane TagashiraDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Karin UrataGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Keitaro SatohDepartment of Pharmacology, Meikai University School of Dentistry, Sakado, Japan.
Naruki FujimotoLaboratory of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Hiroko SonodaLaboratory of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Masahiro IkedaLaboratory of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Toshiyuki MatsuzakiDepartment of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Maebashi, Japan.
Kazuo NishiyamaDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Hisato KunitakeDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Y O GotoBiolabo Co., Ltd., Kobe, Japan.
Masao YamasakiDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
University of Miyazaki · JPAsahi University · JPGunma University · JPMeikai University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimTears secreted from the lacrimal gland are essential for preserving the ocular surface. Thus, dysfunction of the lacrimal gland in Sjögren's syndrome (SS) can lead to dry eye, resulting in a reduced quality of life. We previously reported that blueberry 'leaf' water extract prevents lacrimal hyposecretion in male non-obese diabetic (NOD) mice in a SS-like model. In this study, we investigated the effect of blueberry 'stem' water extract (BStEx) on lacrimal hyposecretion in NOD mice. MATERIALS AND

methodsMale NOD mice were fed 1% BStEx or control (AIN-93G) for 2, 4, or 6 weeks from 4 weeks of age. Pilocarpine-induced tear secretion was measured using a phenol red-impregnated thread. The lacrimal glands were histologically evaluated by HE staining. Inflammatory cytokine levels in the lacrimal glands were measured using ELISA. Immunostaining was performed to examine aquaporin 5 (AQP5) localization. The expression levels of autophagy-related proteins, AQP5, and phosphorylated AMPK were measured using western blotting.

resultsAfter feeding BStEx to mice for 4 or 6 weeks, tear volume was observed to have increased in the BStEx group compared with that in the control group. There were no significant differences in inflammatory cell infiltration, autophagy-related protein expression, or the localization and expression of AQP5 in the lacrimal glands between the two groups. In contrast, AMPK phosphorylation increased in the BStEx group.

conclusionBStEx prevented lacrimal hyposecretion in the SS-like model of male NOD mice, probably by opening tight junctions via the activation of AMPK in lacrimal acinar cells.

Indexed as

Blueberry PlantsDiabetes Mellitus, ExperimentalLacrimal ApparatusSjogren's SyndromeAMP-Activated Protein KinasesAnimalsDisease Models, AnimalMaleMiceMice, Inbred NODPlant ExtractsQuality of LifeAMP-Activated Protein KinasesPlant ExtractsAMPK phosphorylationBlueberry Stemlacrimal glandNOD miceSjögren’s syndrometear secretion

Identifiers

PMID37103112
PMCPMC10188039
OpenAlexW4367173788

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.