ArticleMicrobiology spectrum2023
Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats.
Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed.
- Article
- Detection to Disruption: A Comprehensive Review of Bacterial Biofilms and Therapeutic Advances.Antibiotics (Basel, Switzerland) · 2026Review
- Spatiotemporal Variations in Microbial Community Structure and Assembly Mechanisms Within Recirculating Aquaculture Systems for Mandarin Fish (Microorganisms · 2026Article
- Integrated analysis of gut microbiota structure and metabolic function in patients with cirrhosis and refractory ascites.Biomedical reports · 2026Article
- HVRLocator: a computationally efficient tool for identifying hypervariable regions in large 16S rRNA datasets.GigaScience · 2026Article
- Bacteriophage-mediated cell lysis externalizes a metabolically valuable nutrient to broadly modulate bacterial communities.The ISME journal · 2026Article
- PRIME: a database for 16S rRNA microbiome data with phenotypic reference and comprehensive metadata.Nucleic acids research · 2026Article
- PUDU (pipeline for universal diversity unveiling): an accessible end-to-end workflow for taxonomic profiling and ecological visualization of environmental microbiomes across amplicon, shotgun, and long-read sequencing.Frontiers in bioinformatics · 2026Article
- Review
- Comparison of 16S rRNA gene amplicon and whole-genome shotgun metagenomic sequencing for subgingival oral microbiome profiling.Journal of oral microbiology · 2026Article
- GGut microbes · 2025Review
- Impact of dietary composition on behavioural expression and gut microbiome dynamics in zebrafish.Pflugers Archiv : European journal of physiology · 2025Article
- Exploring potential biomarkers in foods of animal origin.Journal of food science and technology · 2025Review
- Why Are Long-Read Sequencing Methods Revolutionizing Microbiome Analysis?Microorganisms · 2025Review
- Short Read Lengths Recover Ecological Patterns in 16S rRNA Gene Amplicon Data.Molecular ecology resources · 2025Article
- The Role of Microbiota in the Pathogenesis of Bullous Pemphigoid and Pemphigus Vulgaris: Evidence, Controversies, and Perspectives.International journal of molecular sciences · 2025Review
- Microbial Community Distribution in Low Permeability Reservoirs and Their Positive Impact on Enhanced Oil Recovery.Microorganisms · 2025Article
- Impact of Phage Therapy on Pseudomonas syringae pv. syringae and Plant Microbiome Dynamics Through Coevolution and Field Experiments.Environmental microbiology · 2025Article
- The effect of ocean alkalinity enhancement on pelagic bacterial communities: focus points derived from a mesocosm experiment.Frontiers in microbiomes · 2025Article
- Leveraging human microbiomes for disease prediction and treatment.Trends in pharmacological sciences · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 16S rRNA gene works as a rapid and effective marker for the identification of microorganisms in complex communities; hence, a huge number of microbiomes have been surveyed by 16S amplicon-based sequencing. The resolution of the 16S rRNA gene is always considered only at the genus level; however, it has not been verified on a wide range of microbes yet. To fully explore the ability and potential of the 16S rRNA gene in microbial profiling, here, we propose Qscore, a comprehensive method to evaluate the performance of amplicons by integrating the amplification rate, multitier taxonomic annotation, sequence type, and length. Our
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.