Evidence map›Paper›PMID 37101605›Full record

ArticleACS bio & med chem Au2023

A Fluorescence-Based Assay to Probe Inhibitory Effect of Fructose Mimics on GLUT5 Transport in Breast Cancer Cells.

Natasha Rana, Marwa A Aziz, Rabah A T Serya, Deena S Lasheen, Nermin Samir, Frank Wuest, Khaled A M Abouzid, F G West

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS bio & med chem Au, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. GLUT5-overexpression-related tumorigenic implications.Molecular medicine (Cambridge, Mass.) · 2024
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Natasha RanaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Marwa A AzizDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Rabah A T SeryaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Abassia, P.O. Box 11566, Cairo 11566, Egypt.
Deena S LasheenDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Abassia, P.O. Box 11566, Cairo 11566, Egypt.
Nermin SamirDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Abassia, P.O. Box 11566, Cairo 11566, Egypt.
Frank WuestDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.ORCID https://orcid.org/0000-0002-6705-6450
Khaled A M AbouzidDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Abassia, P.O. Box 11566, Cairo 11566, Egypt.
F G WestDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.ORCID https://orcid.org/0000-0001-7419-2314
Ain Shams University · EGUniversity of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid cell division and reprogramming of energy metabolism are two crucial hallmarks of cancer cells. In humans, hexose trafficking into cancer cells is mainly mediated through a family of glucose transporters (GLUTs), which are facilitative transmembrane hexose transporter proteins. In several breast cancers, fructose can functionally substitute glucose as an alternative energy supply supporting rapid proliferation. GLUT5, the principal fructose transporter, is overexpressed in human breast cancer cells, providing valuable targets for breast cancer detection as well as selective targeting of anticancer drugs using structurally modified fructose mimics. Herein, a novel fluorescence assay was designed aiming to screen a series of C-3 modified 2,5-anhydromannitol (2,5-AM) compounds as d-fructose analogues to explore GLUT5 binding site requirements. The synthesized probes were evaluated for their ability to inhibit the uptake of the fluorescently labeled d-fructose derivative 6-NBDF into EMT6 murine breast cancer cells. A few of the compounds screened demonstrated highly potent single-digit micromolar inhibition of 6-NBDF cellular uptake, which was substantially more potent than the natural substrate d-fructose, at a level of 100-fold or more. The results of this assay are consistent with those obtained from a previous study conducted for some selected compounds against

Identifiers

PMID37101605
PMCPMC10125380
OpenAlexW4308411115

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.