ArticleThe Journal of biological chemistry2023
Ferric uptake regulator (Fur) binds a [2Fe-2S] cluster to regulate intracellular iron homeostasis in Escherichia coli.
Article in The Journal of biological chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 36 citations in OpenAlex.
- From the Cosmos to the Cell: The Central Role of Iron in the Chemistry and Evolution of Life.International journal of molecular sciences · 2026Review
- Regulating microbial cell factories to resist ROS stress in chemical production.Archives of microbiology · 2026Review
- TheMicroorganisms · 2026Article
- Glutamine-hydrolyzing GMP Synthase Gene (guaA) Regulates Siderophore Synthesis in the Nitrogen-fixing Bacterium (NFB) Kosakonia radicincitans GXGL-4A.Current microbiology · 2026Article
- Contrasting microbial iron metabolism in sediments from oxic and hypoxic estuaries.Frontiers in microbiology · 2026Article
- The CRISPR/Cas-associated scaRNA modulatesmicroLife · 2026Article
- The dual implications of iron homeostasis as a bacterial threat: its roles in virulence and antimicrobial resistance.Archives of microbiology · 2025Review
- Iron-sulfur cluster biogenesis and regulation of intracellular iron homeostasis in Escherichia coli.Metallomics : integrated biometal science · 2025Review
- Coordination of the Fe-S cluster biogenesis network by the sRNA RyhB inRNA biology · 2025Article
- The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification inVirulence · 2025Article
- Regulatory mechanism of ferrous ion in enhancing the biosynthesis of L-homoserine, L-threonine, and L-isoleucine in Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Article
- Investigating the Antimicrobial Efficacy of Cannabinoids and Their Derivatives AgainstBiology · 2025Article
- Insights into the phylogenetic profile and virulence-associated genes of multidrug-resistant uropathogenicIJID regions · 2025Article
- Iron-mediated post-transcriptional regulation in Toxoplasma gondii.PLoS pathogens · 2025Article
- Article
- EF-hand calcium sensor, EfhP, controls transcriptional regulation of iron uptake by calcium inmBio · 2024Article
- Article
- Effect of HArchives of microbiology · 2024Article
- Regulatory mechanism of C4-dicarboxylates in cyclo (Phe-Pro) production.Microbial cell factories · 2024Article
- Repeated glucose oscillations in high cell-density cultures influence stress-related functions ofPNAS nexus · 2024Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular iron homeostasis in bacteria is primarily regulated by ferric uptake regulator (Fur). It has been postulated that when intracellular free iron content is elevated, Fur binds ferrous iron to downregulate the genes for iron uptake. However, the iron-bound Fur had not been identified in any bacteria until we recently found that Escherichia coli Fur binds a [2Fe-2S] cluster, but not a mononuclear iron, in E. coli mutant cells that hyperaccumulate intracellular free iron. Here, we report that E. coli Fur also binds a [2Fe-2S] cluster in wildtype E. coli cells grown in M9 medium supplemented with increasing concentrations of iron under aerobic growth conditions. Additionally, we find that binding of the [2Fe-2S] cluster in Fur turns on its binding activity for specific DNA sequences known as the Fur-box and that removal of the [2Fe-2S] cluster from Fur eliminates its Fur-box binding activity. Mutation of the conserved cysteine residues Cys-93 and Cys-96 to Ala in Fur results in the Fur mutants that fail to bind the [2Fe-2S] cluster, have a diminished binding activity for the Fur-box in vitro, and are inactive to complement the function of Fur in vivo. Our results suggest that Fur binds a [2Fe-2S] cluster to regulate intracellular iron homeostasis in response to elevation of intracellular free iron content in E. coli cells.
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