ArticleApplied microbiology and biotechnology2023
Dry matter content and inoculant alter the metabolome and bacterial community of alfalfa ensiled at high temperature.
Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 24 citations in OpenAlex.
- Dynamics of fermentation, bacterial community, and nutritional value in alfalfa-sunflower mixed silage with different additives.BMC microbiology · 2026Article
- Effect of Storage Time on the Fermentation Quality, Bacterial Community Structure, and Metabolic Profiles of Jinmu Grain Grass Silage.Microorganisms · 2025Article
- High temperatures and antibacterial plant additives change the fermentation quality, free amino acids and lactic acid bacteria fermentation type in Caragana Korshinskii silage.BMC microbiology · 2025Article
- Involvement of 4-pentenoic acid in causing quality deterioration of nettle silage: study of antibacterial mechanism.Microbiology spectrum · 2025Article
- Jujube powder supplementation optimized high-moisture alfalfa silage through regulating microbial community.Frontiers in microbiology · 2025Article
- Rumen-Degradable Starch Improves Rumen Fermentation, Function, and Growth Performance by Altering Bacteria and Its Metabolome in Sheep Fed Alfalfa Hay or Silage.Animals : an open access journal from MDPI · 2024Article
- Revealing the regulatory impact of nutrient on the production of (R)-2-(4-Hydroxyphenoxy)propanoic acid by Beauveria bassiana biofilms through comparative transcriptomics analyse.Bioprocess and biosystems engineering · 2024Article
- Using multi-omics to explore the effect of Bacillus velezensis SAAS-63 on resisting nutrient stress in lettuce.Applied microbiology and biotechnology · 2024Article
- Symbiotic efficiency of Rhizobium leguminosarum sv. trifolii strains originating from the subpolar and temperate climate regions.Scientific reports · 2024Article
- Effects of inoculation and dry matter content on microbiome dynamics and metabolome profiling of sorghum silage.Applied microbiology and biotechnology · 2024Article
- Characterization of phyllosphere endophytic lactic acid bacteria reveals a potential novel route to enhance silage fermentation quality.Communications biology · 2024Article
- Influence on the fermentation quality, microbial diversity, and metabolomics in the ensiling of sunflower stalks and alfalfa.Frontiers in plant science · 2024Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa silage fermentation quality, metabolome, bacterial interactions, and successions as well as their predicted metabolic pathways were explored under different dry matter contents (DM) and lactic acid bacteria (LAB) inoculations. Silages were prepared from alfalfa with DM contents of 304 (LDM) and 433 (HDM) g/kg fresh weight and inoculated with Lactiplantibacillus plantarum (L. plantarum, LP), Pediococcus pentosaceus (P. pentosaceus, PP), or sterile water (control). The silages were stored at a simulated hot climate condition (35°C) and sampled at 0, 7, 14, 30, and 60 days of fermentation. The results revealed that HDM significantly improved the alfalfa silage quality and altered microbial community composition. The GC-TOF-MS analysis discovered 200 metabolites in both LDM and HDM alfalfa silage, mainly consisting of amino acids, carbohydrates, fatty acids, and alcohols. Compared with LP and control, PP-inoculated silages had increased concentrations of lactic acid (P < 0.05) and essential amino acids (threonine and tryptophan) as well as decreased pH, putrescine content, and amino acid metabolism. However, alfalfa silage inoculated with LP had higher proteolytic activities than control and PP-inoculated silage, as revealed by a higher concentration of ammonia nitrogen (NH
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