Evidence map›Paper›PMID 37099057›Full record

ArticleApplied microbiology and biotechnology2023

Improving tyrosol production efficiency through shortening the allosteric signal transmission distance of pyruvate decarboxylase.

Huanhuan Xu, Bicheng Yu, Wanqing Wei, Xiulai Chen, Cong Gao, Jia Liu, Liang Guo, Wei Song, Liming Liu, Jing Wu

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Huanhuan XuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Bicheng YuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Wanqing WeiState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Xiulai ChenState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Cong GaoState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Jia LiuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Liang GuoState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Wei SongSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
Liming LiuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Jing WuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China. wujing@jiangnan.edu.cn.ORCID http://orcid.org/0000-0001-6911-2041
Jiangnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tyrosol is an important chemical in medicine and chemical industries, which can be synthesized by a four-enzyme cascade pathway constructed in our previous study. However, the low catalytic efficiency of pyruvate decarboxylase from Candida tropicalis (CtPDC) in this cascade is a rate-limiting step. In this study, we resolved the crystal structure of CtPDC and investigated the mechanism of allosteric substrate activation and decarboxylation of this enzyme toward 4-hydroxyphenylpyruvate (4-HPP). In addition, based on the molecular mechanism and structural dynamic changes, we conducted protein engineering of CtPDC to improve decarboxylation efficiency. The conversion of the best mutant, CtPDC

Indexed as

Phenylethyl AlcoholPyruvate DecarboxylaseProtein Engineering4-hydroxyphenylethanolPhenylethyl AlcoholPyruvate DecarboxylaseAllosteric regulationMolecular dynamics simulationPyruvate decarboxylase,·Protein engineeringTyrosol

Identifiers

PMID37099057
OpenAlexW4367048252

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.