Evidence map›Paper›PMID 37096986›Full record

ArticleThe journal of physical chemistry. B2023

Sequential Two-Photon Delayed Fluorescence Anisotropy for Macromolecular Size Determination.

Yi-Han Lu, Matthew C Jenkins, Katherine G Richardson, Sayan Palui, Md Shariful Islam, Jagnyaseni Tripathy, M G Finn, Robert M Dickson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physical chemistry. B, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yi-Han LuSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.ORCID 0000-0002-8436-9475
Matthew C JenkinsSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.
Katherine G RichardsonSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.
Sayan PaluiSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.ORCID 0009-0009-1830-2617
Md Shariful IslamSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.
Jagnyaseni TripathySchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.
M G FinnSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.ORCID 0000-0001-8247-3108
Robert M DicksonSchool of Chemistry and Biochemistry and Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States.ORCID 0000-0003-0042-6194

Funding

Background-free molecular imaging using modulated photoacoustics and targeted contrast agentR01EB028916 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M, EMELIANOV, STANISLAV Y · 2020 to 2023
$2.4M
NIBIB NIH HHS R01 EB028916
6 · The paper itself

Abstract

Time-resolved fluorescence anisotropy (FA) uses the fluorophore depolarization rate to report on rotational diffusion, conformation changes, and intermolecular interactions in solution. Although FA is a rapid, sensitive, and nondestructive tool for biomolecular interaction studies, the short (∼ns) fluorescence lifetime of typical dyes largely prevents the application of FA on larger macromolecular species and complexes. By using triplet shelving and recovery of optical excitation, we introduce optically activated delayed fluorescence anisotropy (OADFA) measurements using sequential two-photon excitation, effectively stretching fluorescence anisotropy measurement times from the nanosecond scale to hundreds of microseconds. We demonstrate this scheme for measuring slow depolarization processes of large macromolecular complexes, derive a quantitative rate model, and perform Monte Carlo simulations to describe the depolarization process of OADFA at the molecular level. This setup has great potential to enable future biomacromolecular and colloidal studies.

Identifiers

PMID37096986
PMCPMC10165651

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.