ArticleBMC oral health2023
Pre-treatment oral microbiome analysis and salivary Stephan curve kinetics in white spot lesion development in orthodontic patients wearing fixed appliances. A pilot study.
Article in BMC oral health, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.
- Orthodontic treatment and oral microbiota changes: a systematic review of oral dysbiosis revealed by 16S rRNA gene analysis.The Angle orthodontist · 2025Pooled it
- Orthodontic Treatment-Induced Periodontal, Microbiological, and Local Inflammatory Changes: A Systematic Review and Meta-Analysis.Biomedicines · 2026Review
- Environmental Stress Induces Altered Composition of Streptococcus mutans Membrane Vesicles: pH-Driven Changes in Membrane Vesicle Production and Composition.Molecular oral microbiology · 2026Article
- A prospective cohort study exploring salivary microbial predictors of white spot lesions during fixed orthodontic treatment.Journal of oral microbiology · 2026Article
- Salivary pH within multifactorial caries risk assessment in children: observational clinical evidence using the Cariogram platform.Frontiers in oral health · 2026Article
- Bacterial Colonization of Orthodontic Devices (Molar Bands, Nance Buttons, and Acrylic Plates) and Its Impact on the Marginal Periodontium and Palatal Fibromucosa in Teenagers: A Cross-Sectional Clinical-Microbiological Study.Medicina (Kaunas, Lithuania) · 2025Article
- Microbiome variability and role ofJournal of oral microbiology · 2025Article
- Dynamics of the oral microbiome during orthodontic treatment and antimicrobial advances for orthodontic appliances.iScience · 2024Review
- A deep learning feature importance test framework for integrating informative high-dimensional biomarkers to improve disease outcome prediction.Briefings in bioinformatics · 2024Article
- Antimicrobial Efficacy of Chemomechanical Carie Removal Agents-A Systematic Integrative Review.Biomedicines · 2024Review
- Effectiveness of fluoride mouthrinse in prevention of demineralization during fixed orthodontic treatment: A systematic review and meta-analysis.Journal of orthodontic science · 2024Review
- Application of an Antibacterial Coating LayerIET nanobiotechnology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhite spot lesions (WSLs) are a formidable challenge during orthodontic treatment, affecting patients regardless of oral hygiene. Multifactorial in nature, amongst potential contributors to their development are the microbiome and salivary pH. The aim of our pilot study is to determine if pre-treatment differences in salivary Stephan curve kinetics and salivary microbiome features correlate with WSL development in orthodontic patients with fixed appliances. We hypothesize that non-oral hygiene determined differences in saliva could be predictive of WSL formation in this patient population through analysis of salivary Stephan curve kinetics, and that these differences would further manifest as changes in the oral microbiome.
methodsIn this prospective cohort study, twenty patients with initial simplified oral hygiene index scores of "good" that were planning to undergo orthodontic treatment with self-ligating fixed appliances for at least 12 months were enrolled. At pre-treatment stage, saliva was collected for microbiome analysis, and at 15-minute intervals after a sucrose rinse over 45 min for Stephan curve kinetics.
results50% of patients developed a mean 5.7 (SEM: 1.2) WSLs. There were no differences in saliva microbiome species richness, Shannon alpha diversity or beta diversity between the groups. Capnocytophaga sputigena exclusively and Prevotella melaninogenica predominantly were found in WSL patients, while Streptococcus australis was negatively correlated with WSL development. Streptococcus mitis and Streptococcus anginosus were primarily present in healthy patients. There was no evidence to support the primary hypothesis.
conclusionsWhile there were no differences in salivary pH or restitution kinetics following a sucrose challenge and no global microbial differences in WSL developers, our data showed change in salivary pH at 5 min associated with an abundance of acid-producing bacteria in saliva. The results suggest salivary pH modulation as a management strategy to inhibit the abundance of caries initiators. Our study may have uncovered the earliest predecessors to WSL/caries development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.