ArticleThe EMBO journal1986
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
Article in The EMBO journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 237 citations in OpenAlex.
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Mechanistic basis for multidrug resistance and collateral drug sensitivity conferred to the malaria parasite by polymorphisms in PfMDR1 and PfCRT.PLoS biology · 2022Article
- Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease-The Example of C9orf72 ALS.International journal of molecular sciences · 2021Review
- The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division.Nature communications · 2021Article
- Article
- Non-equivalence of nuclear import among nuclei in multinucleated skeletal muscle cells.Journal of cell science · 2018Article
- Cytoplasmic transport and nuclear import of plasmid DNA.Bioscience reports · 2017Review
- Iron is a substrate of theThe Journal of biological chemistry · 2017Article
- Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- RNA localization to the Balbiani body in Xenopus oocytes is regulated by the energy state of the cell and is facilitated by kinesin II.RNA (New York, N.Y.) · 2009Article
- NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes.The Journal of cell biology · 2006Article
- Postmitotic nuclear retention of episomal plasmids is altered by DNA labeling and detection methods.Molecular therapy : the journal of the American Society of Gene Therapy · 2005Article
- Optical recording of signal-mediated protein transport through single nuclear pore complexes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The influence of huntingtin protein size on nuclear localization and cellular toxicity.The Journal of cell biology · 1998Article
- Involvement of p62 nucleoporin in angiotensin II-induced nuclear translocation of STAT3 in brain neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Nuclear import and export of influenza virus nucleoprotein.Journal of virology · 1997Article
- Nuclear import of U snRNPs requires importin beta.The EMBO journal · 1997Article
- A novel class of RanGTP binding proteins.The Journal of cell biology · 1997Article
- Identification of different roles for RanGDP and RanGTP in nuclear protein import.The EMBO journal · 1996Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA (from bacteriophage lambda or Xenopus) is assembled into nucleus-like structures when mixed with an extract from Xenopus eggs. Electron microscopy shows that these in vitro-reconstituted nuclei possess complete double membranes; some, but not all, nuclei have pore complexes. Extracts depleted of their endogenous ATP (by addition of ATPases) cannot assemble nuclear envelopes visible by phase-contrast microscopy. Once synthetic nuclei are assembled, however, they are stable when ATP is subsequently depleted, although their chromatin becomes condensed. About one-fourth of the nuclei assembled in vitro from lambda DNA accumulate nuclear proteins such as nucleoplasmin. ATP depletion blocks nucleoplasmin accumulation both in vitro, in pre-assembled synthetic nuclei, and in vivo, in the nucleus of microinjected oocytes. However, nucleoplasmin previously accumulated by reconstituted nuclei or by the germinal vesicle in microinjected oocytes is retained after ATP depletion.
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