ArticleJournal of proteome research2023
Sample Preparation Methods for Targeted Single-Cell Proteomics.
Article in Journal of proteome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Cell-Type-Resolved Proteomics Reveals Distinct Immune and Metabolic Programs Between Primary and Recurrent Ascites in HGSOC.Proteomics. Clinical applications · 2026Article
- Article
- MiProChip: A Scalable Microfluidic Platform for Multiplexed Single-Cell Proteomics via Isobaric Labeling.Analytical chemistry · 2026Article
- The pursuit of ultrasensitive phosphoproteomics to unravel signalling in rare cells.Communications biology · 2025Review
- Comparative Proteomics of Salinity Stress Responses in Fish and Aquatic Invertebrates.Proteomics · 2025Review
- Evolving stratification and biomarker discovery in cancer research with technological advancement of proteomics: 35 years and counting.Bioscience reports · 2025Review
- Mass Spectrometry-based Solutions for Single-cell Proteomics.Genomics, proteomics & bioinformatics · 2025Review
- Trends in Mass Spectrometry-Based Single-Cell Proteomics.Analytical chemistry · 2025Review
- FISH-FACS proteomics: enhanced label-free quantitative proteome analysis from low cell numbers of uncultured environmental microorganisms.ISME communications · 2025Article
- A critical evaluation of ultrasensitive single-cell proteomics strategies.Analytical and bioanalytical chemistry · 2024Review
- Pick-up single-cell proteomic analysis for quantifying up to 3000 proteins in a Mammalian cell.Nature communications · 2024Article
- EquiCP: Targeted Single-Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Toward Single Bacterium Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
- Easy and Accessible Workflow for Label-Free Single-Cell Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
- Rapid, One-Step Sample Processing for Label-Free Single-Cell Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared three cell isolation and two proteomic sample preparation methods for single-cell and near-single-cell analysis. Whole blood was used to quantify hemoglobin (Hb) and glycated-Hb (gly-Hb) in erythrocytes using targeted mass spectrometry and stable isotope-labeled standard peptides. Each method differed in cell isolation and sample preparation as follows: 1) FACS and automated preparation in one-pot for trace samples (autoPOTS); 2) limited dilution via microscopy and a novel rapid one-pot sample preparation method that circumvented the need for the solid-phase extraction, low-volume liquid handling instrumentation and humidified incubation chamber; and 3) CellenONE-based cell isolation and the same one-pot sample preparation method used for limited dilution. Only the CellenONE device routinely isolated single-cells from which Hb was measured to be 540-660 amol per red blood cell (RBC), which was comparable to the calculated SI reference range for mean corpuscular hemoglobin (390-540 amol/RBC). FACSAria sorter and limited dilution could routinely isolate single-digit cell numbers, to reliably quantify CMV-Hb heterogeneity. Finally, we observed that repeated measures, using 5-25 RBCs obtained from
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