Evidence map›Paper›PMID 37093483›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Isolation of Bacterial Extracellular Vesicles and Identification of Their Protein Cargo.

Roberto Alvarez-Fernandez Garcia, Guillermo Aragoneses-Cazorla, Laura Lerma, Rafael C Prados-Rosales, Jose L Luque-Garcia

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Roberto Alvarez-Fernandez GarciaDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain.
Guillermo Aragoneses-CazorlaDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain.
Laura LermaDepartment of Preventive Medicine and Public Health and Microbiology, Faculty of Medicine, Autonoma University of Madrid, Madrid, Spain.
Rafael C Prados-RosalesDepartment of Preventive Medicine and Public Health and Microbiology, Faculty of Medicine, Autonoma University of Madrid, Madrid, Spain. rafael.prados@uam.es.
Jose L Luque-GarciaDepartment of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain. jlluque@ucm.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial membrane vesicles (BMVs) are important effectors in the pathogenesis, virulence, and biofilm formation during different bacterial infections. Because of their structure, BMVs can be applied as drug delivery systems (DDS) or in the production of immunogenic vaccines against different untreated diseases. In this sense, different antigens or immune stimulator molecules, such as proteins can be extracted for the development of such vaccines. Here, we describe a protocol adapted to be used in mycobacteria, Gram-positive, and Gram-negative bacteria for the isolation of BMVs, and further mass spectrometry-based characterization of their protein cargo.

Indexed as

Bacterial InfectionsExtracellular VesiclesVaccinesBacteriaGram-Negative BacteriaHumansVaccinesBacterial extracellular vesiclesMass spectrometryMycobacteriaProtein cargoProteomicsVesicles isolation

Identifiers

PMID37093483

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.