Evidence map›Paper›PMID 37093473›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture.

Hidefumi Shinohara

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Hidefumi ShinoharaDepartment of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan. shino@fpu.ac.jp.
Fukui Prefectural University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular signals are usually perceived by membrane-localized receptors that transduce intercellular signals to activate various pathways. In plants, single transmembrane receptor kinases act as receptors for extracellular signals. Endogenous secreted peptide hormones have been recognized as novel signaling molecules, functioning through the formation of ligand-receptor pairs in plants. Recently, research on plant peptide hormone-receptor interactions based on the structural biology approach has greatly improved; however, the dissociation constant of recombinant receptor molecules expressed in insect cells using the baculovirus expression system is relatively low. We introduce here a method for creating a stable and functional homogeneous expression system for plant receptor kinases using tobacco BY-2 cells while maintaining conventional ligand-binding activity. This strategy will help improve our understanding of plant endogenous peptide ligand-receptor interactions.

Indexed as

Peptide HormonesPlantsCell CommunicationLigandsReceptors, PeptideSignal TransductionLigandsPeptide HormonesReceptors, PeptideEctodomainHaloTagLigand-receptor pairsPeptide hormonesPlantReceptor kinases

Identifiers

PMID37093473
OpenAlexW4366819860

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.