Evidence map›Paper›PMID 37093470›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS).

Heather McDuffie Sullivan, Ewa Krupinska, Anna Andersson Rasmussen, Juan Manuel Orozco Rodriguez, Wolfgang Knecht

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Heather McDuffie SullivanLund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden.
Ewa KrupinskaLund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden.
Anna Andersson RasmussenLund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden.
Juan Manuel Orozco RodriguezLund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden.
Wolfgang KnechtLund Protein Production Platform (LP3) & Protein Production Sweden (PPS), Department of Biology, Lund University, Lund, Sweden. Wolfgang.Knecht@biol.lu.se.
Lund University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.

Indexed as

BaculoviridaeGenetic VectorsRecombinant ProteinsRecombinant ProteinsBaculovirus-infected insect cells (BIIC)Cell cultureG protein-coupled receptorsInsect cellsProtein complexesProtein kinasesProtein Production Sweden (PPS)Structural biology

Identifiers

PMID37093470
OpenAlexW4366819679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.