Evidence map›Paper›PMID 37093468›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate.

Diana Gomes, Luís A Passarinha, Ângela Sousa

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Diana GomesCICS-UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
Luís A PassarinhaCICS-UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal. lpassarinha@fcsaude.ubi.pt.
Ângela SousaCICS-UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal. angela@fcsaude.ubi.pt.
University of Beira Interior · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cervical cancer has been extensively associated with human papillomavirus (HPV) due to the expression of oncoproteins such as E6. This protein can interfere with p53 tumor suppressor activity, blocking apoptosis of abnormal cells. The functional inhibition of E6 protein is a promising therapeutic strategy for HPV-induced cancers. Conducting biointeraction and characterization studies between E6 protein and potential anti-HPV drugs is necessary to obtain large quantities of high-purity and soluble E6 protein. The recombinant production of E6 protein is particularly challenging because it tends to aggregate. One way to circumvent this problem is to use a dual MBP-His

Indexed as

Oncogene Proteins, ViralUterine Cervical NeoplasmsEscherichia coliFemaleHumansRecombinant Fusion ProteinsTumor Suppressor Protein p53Oncogene Proteins, ViralRecombinant Fusion ProteinsTumor Suppressor Protein p53Affinity chromatographyCervical cancerE6 proteinFusion proteinHis-tagMaltose-binding proteinProtein expression and purificationSize exclusion chromatographyWestern blot

Identifiers

PMID37093468
OpenAlexW4366822733

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.