ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2023
A ring trial to harmonize Toxoplasma gondii microsatellite typing: comparative analysis of results and recommendations for optimization.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Molecular epidemiology of Toxoplasma gondii in impala (Aepyceros melampus) from the Greater Kruger in South Africa: Detection of the Africa 4 lineage.PLoS neglected tropical diseases · 2026Article
- Evolution ofJournal of parasitology research · 2026Review
- Evolutionary history, longevity and terrestriality predictInternational journal for parasitology. Parasites and wildlife · 2025Article
- Molecular detection ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Seroprevalence ofPathogens (Basel, Switzerland) · 2025Article
- The importance of translational research in the study of ocular toxoplasmosis: insights from the 17th International Congress on Toxoplasmosis 2024.FEMS microbes · 2025Article
- Toxoplasma gondii from Gabonese forest, Central Africa: First report of an African wild strain.PLoS neglected tropical diseases · 2025Article
- Multiple Typing Approach to CharacterizeAnimals : an open access journal from MDPI · 2024Article
- Communicating and disseminating One Health: successes of the One Health European Joint Programme.Journal of medical microbiology · 2024Article
- Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Drivers of infection with Toxoplasma gondii genotype type II in Eurasian red squirrels (Sciurus vulgaris).Parasites & vectors · 2024Article
- First detection ofFrontiers in cellular and infection microbiology · 2024Article
- Fulminant Pneumonia Due to Reactivation of Latent Toxoplasmosis in a Cat-A Case Report.Pathogens (Basel, Switzerland) · 2023Article
- Genetic diversity of Toxoplasma gondii in South America: occurrence, immunity, and fate of infection.Parasites & vectors · 2023Review
- Fatal toxoplasmosis in a captive squirrel monkey (Saimiri boliviensis) in Portugal.Veterinary research communications · 2023Article
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Authors and funding
17 authors.
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Abstract
A ring trial among five European laboratories was organized to reach consistency in microsatellite (MS) typing of the zoonotic parasite Toxoplasma gondii. Three sample sets were circulated and analyzed by each laboratory following a previously published method that is based on fragment length polymorphism of 15 MS markers. The first sample set compared typing results in general and focused on effects of DNA concentration; the second sample set focused on the polymorphic fingerprinting markers that can differentiate T. gondii strains within the same archetypal lineage; and the third set focused on non-archetypal genotypes. Methodological variations between laboratories, including the software programs used to determine MS fragment length, were collated using a questionnaire. Overall, lineage-level typing results reached a high level of agreement, especially in samples with the highest DNA concentrations. However, laboratory-specific differences were observed for particular markers. Major median differences in fragment length, of up to 6 base pairs, were related to the fluorophore used to label fragment-specific primers. In addition, primer pairs with identical sequences obtained from different suppliers resulted in fragments of differing length. Furthermore, differences in the way the sequencing profiles were assessed and interpreted may have led to deviating results in fragment length determination. Harmonization of MS typing, for example, by using the same fluorophores or by numerical adjustments applied to the fragment-lengths determined, could improve the uniformity of the results across laboratories. This is the first interlaboratory comparison, providing guidelines (added as a supplement) for the optimization of this technique.
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