Evidence map›Paper›PMID 37091250›Full record

ArticleiScience2023

Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency.

Qian Ban, Wenqiao Hui, Quanchao Duan, Wenfei Liu, Meng Zhang, Xiaofeng Li, Hanyue Kang, Zhenni Shi, Yan Zhang, Jinbin Hou and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 63% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

Qian BanCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Wenqiao HuiAnhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agriculture Sciences, Hefei, Anhui 230031, China.
Quanchao DuanCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Wenfei LiuCalifornia NanoSystems Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Meng ZhangCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Xiaofeng LiCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Hanyue KangKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 China.
Zhenni ShiCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Yan ZhangCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Jinbin HouCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Xiuzhen XuKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 China.
Shoudong YeCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Baowei ZhangCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China.
Xiaobin XuKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 China.
Anhui University · CNTongji University · CNAnhui Academy of Agricultural Sciences · CNCalifornia NanoSystems Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (μPyA/+2i) inserts for use in commercial multi-well plates, as the alternative cultivation platform for maintaining long-term self-renewal and pluripotency of multiple mESCs and mouse induced pluripotent stem cells. Relevant evidence including cell morphology characterization increased alkaline phosphatase activity, high expression of mESC self-renewal markers, decreased levels of differentiation-associated markers, and high proportion of self-renewal marker cells are provided. Further studies demonstrated that μPyA/+2i could cause a higher cell density in mESC colony, and induce gene expression changes. Subsequent studies showed that μPyA/+2i can influence the cytoskeleton and promote cell adhesion through

Indexed as

BiomaterialsStem cells research

Identifiers

PMID37091250
PMCPMC10119598
OpenAlexW4327703890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.